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Administration of growth hormone and nandrolone decanoate alters mRNA expression of the GABAB receptor subunits as well as of the GH receptor, IGF-1, and IGF-2 in rat brain
- Source :
- Growth Hormone & IGF Research. 24:60-66
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Objective The illicit use of anabolic androgenic steroids (AAS), especially among young adults, is of major concern. Among AAS users it is common to combine the AAS nandrolone decanoate (ND), with intake of growth hormone (GH) and a connection between gonadal steroids and the GH system has been suggested. Both AAS and GH affect functions in the brain, for example those associated with the hypothalamus and pituitary, and several GH actions are mediated by growth factors such as insulin-like growth factor 1 (IGF-1) and insulin-like growth factor 2 (IGF-2). The GABAergic system is implicated in actions induced by AAS and previous studies have provided evidence for a link between GH and GABA B receptors in the brain. Our aim was to examine the impact of AAS administration and a subsequent administration of GH, on the expression of GABA B receptors and important GH mediators in rat brain. Design The aim was to investigate the CNS effects of a high-dose ND, and to study if a low, but physiological relevant, dose of GH could reverse the ND-induced effects. In the present study, male rats were administered a high dose of ND every third day during three weeks, and subsequently the rats were given recombinant human GH (rhGH) during ten days. Quantitative PCR (qPCR) was used to analyze gene expression in hypothalamus, anterior pituitary, caudate putamen, nucleus accumbens, and amygdala. Results In the pituitary gland, the expression of GABA B receptor subunits was affected differently by the steroid treatment; the GABA B1 mRNA expression was decreased whereas a distinct elevation of the GABA B2 expression was found. Administration of ND also caused a decrease of GHR, IGF-1, and IGF-2 mRNA expression in the pituitary while the corresponding expression in the hypothalamus, caudate putamen, nucleus accumbens, and amygdala was unaffected. The rhGH administration did not alter the GABA B2 expression but increased the GABA B1 gene expression in the hypothalamus as compared to the AAS treated group. Conclusions These results provide new insights on the impact of ND and GH on the brain and highlight the interaction of these hormones with systems influencing GABA B receptor expression. The physiological significance of the observed effects of these hormones is discussed.
- Subjects :
- Male
medicine.medical_specialty
Pituitary gland
Injections, Subcutaneous
Endocrinology, Diabetes and Metabolism
Growth hormone receptor
Biology
Nucleus accumbens
Pharmacology
Anabolic Agents
Endocrinology
Anterior pituitary
Insulin-Like Growth Factor II
Internal medicine
medicine
Animals
Humans
Nandrolone
RNA, Messenger
Insulin-Like Growth Factor I
Rats, Wistar
Receptor
Human Growth Hormone
Brain
Receptors, Somatotropin
Recombinant Proteins
Rats
Protein Subunits
medicine.anatomical_structure
Gene Expression Regulation
Receptors, GABA-B
nervous system
Nandrolone Decanoate
Hypothalamus
GABAergic
Hormone
Subjects
Details
- ISSN :
- 10966374
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Growth Hormone & IGF Research
- Accession number :
- edsair.doi.dedup.....a67074410bb5a3054d44534722b3253f