Back to Search
Start Over
Genome-wide identification of glutathione peroxidase (GPX) gene family and their response to abiotic stress in cucumber
- Source :
- 3 Biotech. 8(3)
- Publication Year :
- 2017
-
Abstract
- Plant glutathione peroxidases (GPXs) are non-heme thiol peroxidases that play vital roles in maintaining H(2)O(2) homeostasis and regulating plant response to abiotic stress. Here, we performed a comparative genomic analysis of the GPX gene family in cucumber (Cucumis sativus). As a result, a total of 6 CsGPX genes were identified, which were unevenly located in four out of the seven chromosomes in cucumber genome. Based on the phylogenetic analysis, the GPX genes of cucumber, Arabidopsis and rice could be classified into five groups. Analysis of the distribution of conserved domains of GPX proteins showed that all these proteins contain three highly conserved motifs, as well as other conserved sequences and residues. Gene structure analysis revealed a conserved exon–intron organization pattern of these genes. Through analyzing the promoter regions of CsGPX genes, many hormone-, stress-, and development-responsive cis-elements were identified. Moreover, we also investigated their expression patterns in different tissues and developmental stages as well as in response to abiotic stress and x acid (ABA) treatments. The qRT-PCR results showed that the transcripts of CsGPX genes varied largely under abiotic stress and ABA treatments at different time points. These results demonstrate that cucumber GPX gene family may function in tissue development and plant stress responses. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13205-018-1185-3) contains supplementary material, which is available to authorized users.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Genetics
biology
Abiotic stress
Environmental Science (miscellaneous)
biology.organism_classification
01 natural sciences
Agricultural and Biological Sciences (miscellaneous)
Genome
Conserved sequence
03 medical and health sciences
030104 developmental biology
Arabidopsis
biology.protein
Gene family
Original Article
Cucumis
Gene
010606 plant biology & botany
Biotechnology
Peroxidase
Subjects
Details
- ISSN :
- 2190572X
- Volume :
- 8
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- 3 Biotech
- Accession number :
- edsair.doi.dedup.....a666cc87bbd40687520951b05183c3ee