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Pectin/chitosan/tripolyphosphate encapsulation protects the rat lung from fibrosis and apoptosis induced by paraquat inhalation

Pectin/chitosan/tripolyphosphate encapsulation protects the rat lung from fibrosis and apoptosis induced by paraquat inhalation

Authors :
Behroz Davari
Ebrahim Mohammadi
Naser Pajouhi
Afshin Maleki
Marzieh Rashidipour
Bahram Rasoulian
Source :
Pesticide Biochemistry and Physiology. 178:104919
Publication Year :
2021
Publisher :
Elsevier BV, 2021.

Abstract

Background Paraquat poisoning leads to lung injury and pulmonary fibrosis. The effect of paraquat encapsulation by previously described Pectin/Chitosan/Tripolyphosphate nanoparticles on its pulmonary toxicity was investigated in present study in a rat model of poison inhalation. Material and method The rats inhaled nebulized different formulation of paraquat (n = 5) for 30 min in various experimental groups. Lung injury and fibrosis scores, Lung tissue enzymatic activities, apoptosis markers were determined compared among groups. Results Encapsulation of paraquat significantly rescued both lung injury and fibrosis scores. Lung MDA level was reduced by encapsulation. Paraquat poisoning led to lung tissue apoptosis as was evidenced by higher Caspase-3 and Bax/Bcl2 expressions in rats subjected to paraquat inhalation instead of normal saline or free nanoparticles. Again, nanoencapsulation reduced these apoptosis markers significantly. Alpha-SMA expression was also reduced by encapsulation. Nanoparticles per se have no or little toxicity as was evidenced by inflammatory and apoptotic markers and histological scores. Conclusion In a rat model of inhalation toxicity of paraquat, loading of this herbicide on PEC/CS/TPP nanoparticles reduced acute lung injury and fibrosis. The encapsulation also led to lower apoptosis, oxidative stress and alpha-SMA expression in the lung tissue.

Details

ISSN :
00483575
Volume :
178
Database :
OpenAIRE
Journal :
Pesticide Biochemistry and Physiology
Accession number :
edsair.doi.dedup.....a664cd40bed7beea5db4b5730b06225f
Full Text :
https://doi.org/10.1016/j.pestbp.2021.104919