Back to Search
Start Over
Role of Phosphatidylinositol Kinase in PDGF Receptor Signal Transduction
- Source :
- Science. 243:1191-1194
- Publication Year :
- 1989
- Publisher :
- American Association for the Advancement of Science (AAAS), 1989.
-
Abstract
- The molecules with which the platelet-derived growth factor (PDGF) receptor interacts to elicit the biochemical reactions responsible for cell proliferation have not been identified. Antisera directed against specific PDGF receptor peptides coprecipitated a phosphatidylinositol (PI) kinase and the PDGF receptor. Immunoprecipitates from PDGF-stimulated cells contained 10 to 50 times as much PI kinase as those from unstimulated cells. Mutation of the PDGF receptor by deletion of its kinase insert region resulted in a receptor markedly less effective than the wild type in eliciting cell proliferation and defective in PDGF-stimulated PI kinase, but still capable of PDGF-induced receptor autophosphorylation and phosphoinositide hydrolysis. These data show that the PDGF receptor is physically associated with a PDGF-sensitive PI kinase that is distinct from tyrosine kinase and is not required for PDGF-induced PI hydrolysis. The finding that the mutant PDGF receptor missing the kinase insert domain elicited known early biochemical responses to PDGF, but did not associate with or regulate PI kinase, suggests a novel role for the receptor-associated PI kinase in the transmission of mitogenic signals.
- Subjects :
- Receptors, Cell Surface
Mitogen-activated protein kinase kinase
Phosphatidylinositols
Tropomyosin receptor kinase C
Cell Line
Mice
Cricetinae
Animals
Receptors, Platelet-Derived Growth Factor
ASK1
Phosphorylation
Phosphotyrosine
1-Phosphatidylinositol 4-Kinase
Immunosorbent Techniques
Immunoassay
Platelet-Derived Growth Factor
Chromatography
Mice, Inbred BALB C
Multidisciplinary
MAP kinase kinase kinase
biology
Akt/PKB signaling pathway
Phosphotransferases
Protein-Tyrosine Kinases
Molecular biology
Mutation
ROR1
biology.protein
Tyrosine
Cyclin-dependent kinase 9
Platelet-derived growth factor receptor
Signal Transduction
Subjects
Details
- ISSN :
- 10959203 and 00368075
- Volume :
- 243
- Database :
- OpenAIRE
- Journal :
- Science
- Accession number :
- edsair.doi.dedup.....a66481cfd901ddba0135c4b4386ca62d