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Rotational Rheology of Bovine Serum Albumin Solutions: Confounding Effects of Impurities, Mechanistic Considerations and Potential Implications on Protein Formulation Development
- Source :
- Pharmaceutical research. 35(8)
- Publication Year :
- 2018
-
Abstract
- To show and rationalize the confounding effects on the rotational/oscillatory rheology of surface active impurities in commercial protein formulations such as bovine serum albumin, BSA. Bulk and interfacial rotational/oscillatory rheology were used to study the viscosity, complex viscosity, storage/elastic modulus, G’ and loss/viscous modulus, G”, as a function of time of aqueous formulations of BSA and their purified components. Viscosity/time profiles at steady shear for different commercial BSA products and lots showed viscosity increase, decrease and time-independent profiles at low shear rates. All lots showed shear thinning. BSA monomer and dimers/aggregates, in general, showed similar profiles. Addition of low levels of surfactant or high shear rates rendered all solutions to be Newtonian-like. Interfacial viscosity studies paralleled those on the rotational rheometer. G’ > G” with viscosity increase and G’
- Subjects :
- Rheometer
Drug Compounding
Pharmaceutical Science
02 engineering and technology
01 natural sciences
Viscosity
Protein Aggregates
Rheology
Pulmonary surfactant
Elastic Modulus
0103 physical sciences
Animals
Pharmacology (medical)
Bovine serum albumin
Elastic modulus
Pharmacology
Shear thinning
010304 chemical physics
Rheometry
biology
Chemistry
Protein Stability
Organic Chemistry
Water
Serum Albumin, Bovine
021001 nanoscience & nanotechnology
Chemical engineering
biology.protein
Molecular Medicine
Cattle
0210 nano-technology
Biotechnology
Subjects
Details
- ISSN :
- 1573904X
- Volume :
- 35
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Pharmaceutical research
- Accession number :
- edsair.doi.dedup.....a6415cc05f81267a39ec43f501e78555