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Hexagonal surface array in a protein-secreting bacterium, Bacillus brevis 47
- Source :
- Biochimica et biophysica acta. 693(1)
- Publication Year :
- 1982
-
Abstract
- Bacillus brevis 47, a protein-secreting bacterium, contained two major proteins with approximate molecular weights of 150 000 and 130 000 in the cell wall. The cell surface was covered with a hexagonally arranged array of six structural units about 4 nm in diameter with a lattice constant of 14.5 nm. The regular array structure as well as the chemical composition of cell envelopes remained the same regardless of the growth conditions. A mutant, strain 47-57, which was isolated as a phage resistant colony, contained only the 150 000 protein as a major cell wall protein. Although the mutant had hexagonally arranged arrays with the same lattice constant as that of wild-type cells, the distribution of mass in the unit cell differed considerably from that of the wild-type cells. The number of structural units in the unit cell of the mutant was reduced from six to three. Taking these results together with filtered images of the wild-type and mutant envelopes, two possible models for the surface array of B. brevis 47 are discussed.
- Subjects :
- Bacillus (shape)
Strain (chemistry)
biology
Molecular mass
Cell
Mutant
Cell Membrane
Biophysics
Bacillus
Cell Biology
biology.organism_classification
Biochemistry
Cell membrane
Cell wall
Molecular Weight
Crystallography
Microscopy, Electron
medicine.anatomical_structure
Lattice constant
Bacterial Proteins
Cell Wall
Mutation
medicine
Electrophoresis, Polyacrylamide Gel
Subjects
Details
- ISSN :
- 00063002
- Volume :
- 693
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biochimica et biophysica acta
- Accession number :
- edsair.doi.dedup.....a6288ba75f21f87cc56c85ee55ba5bf4