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Class III POU Genes of Zebrafish Are Predominantly Expressed in the Central Nervous System
- Source :
- Nucleic Acids Research. 24:4874-4881
- Publication Year :
- 1996
- Publisher :
- Oxford University Press (OUP), 1996.
-
Abstract
- POU genes encode a family of transcription factors involved in a wide variety of cell fate decisions and in the regulation of differentiation pathways. We have searched for POU genes in the zebrafish, a popular model organism for the study of early development of vertebrates. Besides five putative pseudogenes we have identified five POU genes that are expressed during embryogenesis. Probes obtained by PCR were used to isolate full-length cDNAs. Four of the isolated genes encode proteins with class III POU domains. Analysis of genomic clones suggests that the fish genes in general do not contain introns, similar to class III genes of mammals. However, the C-termini of two of the encoded proteins vary due to facultative splicing of a short intervening sequence. These two genes show very strong similarities in their sequence. They have probably arisen by gene duplication, possibly as part of a larger scale duplication of part of the zebrafish genome. Analysis of the expression of the class III genes shows that they are predominantly expressed in the central nervous system and that they may play important roles in patterning the embryonic brain.
- Subjects :
- Central Nervous System
DNA, Complementary
Pseudogene
Molecular Sequence Data
Polymerase Chain Reaction
Genome
Gene duplication
Genetics
Animals
Humans
Amino Acid Sequence
Cloning, Molecular
Zebrafish
Gene
Sequence Homology, Amino Acid
biology
POU domain
Intron
Gene Expression Regulation, Developmental
biology.organism_classification
DNA-Binding Proteins
POU Domain Factors
embryonic structures
RNA splicing
Pseudogenes
Transcription Factors
Research Article
Subjects
Details
- ISSN :
- 13624962 and 03051048
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....a612e4818b32843259c3c13ad01a6ee3