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Molecular techniques and their limitations shape our view of the holobiont
- Source :
- Zoology, 137 . Art.Nr. 125695.
- Publication Year :
- 2019
-
Abstract
- It is now recognised that the biology of almost any organism cannot be fully understood without recognising the existence and potential functional importance of associated microbes. Arguably, the emergence of this holistic viewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNA gene amplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of contexts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evolutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we examine each of these categories of measurement and inference with a focus on the questions they make tractable, and the degree to which their capabilities and limitations shape our view of the holobiont. published
- Subjects :
- 0106 biological sciences
0301 basic medicine
Microbial diversity
Microbiota
Inference
Molecular Technique
Holobiont, Imaging, Metagenomic sequencing, Evolutionary inference, Model system, Multi-omics
Biology
010603 evolutionary biology
01 natural sciences
Holobiont
03 medical and health sciences
030104 developmental biology
Functional importance
Evolutionary biology
ddc:570
Amplicon sequencing
Environmental Microbiology
Animal Science and Zoology
Identification (biology)
Symbiosis
Organism
Subjects
Details
- ISSN :
- 18732720
- Volume :
- 137
- Database :
- OpenAIRE
- Journal :
- Zoology (Jena, Germany)
- Accession number :
- edsair.doi.dedup.....a6121b562b60017c7ed2fd09dd028195