Comparative Analysis of Genomic Data To Determine Codon Usage and Amino Acid Usage in Tropheryma Whipplei

The next-generation sequencing methods assisted the scientific community in creating an enormous amount of genomic data, which is found to be very useful in terms of comparing the structural properties and pathogenicity of various bacterial strains. Tropheryma whipplei causes constitutional diseases like Gastroenteritis, Endocarditis, where aetiology directly depends significantly on genetic sequences. In this research Twist strain and TW 08/27 of T. whipplei were selected for In-silico analysis. As the chosen bacterium is fatal in live form, so to design an effective preventive vaccination, the primary target is to analyze the available Genomic sets. In this research, it has been observed the higher similarity between these two strains in their genomic sets. Intra-strand biasness at codons develops the greater expression of genes mostly due to translational selection. But strand-specific mutational stress was not highly significant at amino acid usage levels. The basic research finding in this study was to determine the preference of GC rich codons in leading strands that were expressing rapidly in comparison to lagging strand in T. whipplei. Also, the characterization of two genomes was achieved by comparing membranous genes, which is responsible for exhibiting significant diversity in strains of T.whipplei.