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Anastomotic healing in a rat model of peritonitis after non-steroidal anti-inflammatory drug administration
- Source :
- European Journal of Histochemistry, Vol 64, Iss 1 (2020), European Journal of Histochemistry : EJH
- Publication Year :
- 2020
- Publisher :
- PAGEPress Publications, 2020.
-
Abstract
- The tissue inflammatory response can influence the outcome of anastomotic healing. Anastomotic leakage represents a dreadful complication after gastrointestinal surgery, in particular sepsis and intra-abdominal infections impair the restorative process of colic anastomoses. It has been debated whether the administration of non-steroidal anti-inflammatory drugs (NSAIDs) is a risk factor for dehiscence, since many patients receive NSAIDs in the early postoperative period. Our aim was, for the first time, to analyze the morpho-functional effects of postoperative administration of two commonly used NSAIDs, Diclofenac and Ketorolac, on the healing process of colo-colic anastomoses constructed under condition of fecal peritonitis in a rat model. Sixty adult male rats underwent two surgical procedures: peritonitis induction and colo-colic anastomosis, and were divided into three groups: 20 rats received saline; 20 rats 4 mg/kg Diclofenac and 20 rats 5 mg/kg Ketorolac. We assessed anastomosis strength, morphological features of tissue wound healing, immunohistochemical metalloproteinase 9 (MMP9) expression and collagen deposition and content by Sirius red staining and hydroxyproline level. We found no significant difference in bursting pressure, collagen content and organization and morphological features between the groups, except a significantly reduced presence of inflammatory cells and MMP9 expression in the groups treated with NSAIDs. Our findings showed that Diclofenac and Ketorolac administration did not affect post-surgical healing and did not increase the leakage risk of colo-colic anastomoses during peritonitis.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
Diclofenac
Histology
colo-colic anastomosis
Biophysics
Peritonitis
Anastomotic Leak
wound healing
Anastomosis
Gastroenterology
Article
Sepsis
03 medical and health sciences
chemistry.chemical_compound
Hydroxyproline
0302 clinical medicine
Risk Factors
Internal medicine
Surgical Wound Dehiscence
medicine
Animals
Rats, Wistar
peritonitis
Cecum
Sirius Red
lcsh:QH301-705.5
Non-steroidal inflammatory drugs
business.industry
MMP9
Anastomosis, Surgical
Anti-Inflammatory Agents, Non-Steroidal
Cell Biology
medicine.disease
digestive system diseases
Ketorolac
030104 developmental biology
Matrix Metalloproteinase 9
chemistry
lcsh:Biology (General)
inflammation
030220 oncology & carcinogenesis
business
Wound healing
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20388306
- Volume :
- 64
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- European Journal of Histochemistry
- Accession number :
- edsair.doi.dedup.....a5f9e726a887c0e4c0457a1be403f061