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Efficient degradation and detoxification of methylene blue dye by a newly isolated ligninolytic enzyme producing bacterium Bacillus albus MW407057
- Source :
- Colloids and surfaces. B, Biointerfaces. 206
- Publication Year :
- 2021
-
Abstract
- In present work, a LiP enzyme producing bacterium was isolated form textile wastewater and sludge sample and identified as Bacillus albus by 16S rRNA gene sequencing analysis. This bacterium decolorized 99.27 % MB dye and removed 83.87 % COD within 6 h at 30 °C, pH 7, 100 rpm and 100 mg/l of dye concentration in presence of glucose and yeast extract as carbon and nitrogen source, respectively. The bacterium also produced LiP enzyme of molecular weight ∼48 kDa, characterized by SDS-PAGE analysis. Different metabolites like monomethylthionine, thionin, (E)-2-(3-Oxopropylidene)-2H-benzo[b][1,4] thiozine-3-carboxylic acid, N-(3,4-dihydroxyphenyl)-N-methylformamide, ethylamine, water and carbon dioxide produced during treatment process were characterized by FT-IR and LC-MS analysis. Further, the toxicity assessment results showed that the toxicity of bacteria treated dye solution was reduced significantly allowing 90 % seed germination indicating that the isolated bacterium B. albus has high potential to decolorize and detoxify MB dye for environmental safety.
- Subjects :
- Bacillus
02 engineering and technology
01 natural sciences
chemistry.chemical_compound
Colloid and Surface Chemistry
RNA, Ribosomal, 16S
0103 physical sciences
Spectroscopy, Fourier Transform Infrared
Yeast extract
Physical and Theoretical Chemistry
Coloring Agents
chemistry.chemical_classification
Chromatography
010304 chemical physics
biology
Bacteria
Surfaces and Interfaces
General Medicine
Lignin peroxidase
021001 nanoscience & nanotechnology
biology.organism_classification
Thionin
Methylene Blue
Enzyme
Biodegradation, Environmental
chemistry
Toxicity
0210 nano-technology
Methylene blue
Biotechnology
Subjects
Details
- ISSN :
- 18734367
- Volume :
- 206
- Database :
- OpenAIRE
- Journal :
- Colloids and surfaces. B, Biointerfaces
- Accession number :
- edsair.doi.dedup.....a5d85ef4ed2b8c0b03e194992a660310