Quantification of hepatitis C virus in human liver and serum samples by using LightCycler reverse transcriptase PCR

A highly sensitive, non-probe-based, real-time quantitative reverse transcriptase PCR was developed for viral load measurements in both serum and liver samples from patients with hepatitis C virus (HCV) infection. With synthetic RNA, the linearity of the approach was conserved over a wide range of HCV copy numbers. There was a strong correlation between hepatic and serum viral load measurements ( r = 0.689, P = 0.004, n = 15), indicating that the level of viremia reflected the amount of virus present in the liver.