Back to Search
Start Over
The pepper patatin-like phospholipase CaPLP1 functions in plant cell death and defense signaling
- Source :
- Plant Molecular Biology. 84:329-344
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- Phospholipases hydrolyze phospholipids into fatty acids and other lipophilic substances. Phospholipid signaling is crucial for diverse cellular processes in plants. However, the precise role of phospholipases in plant cell death and defense signaling is not fully understood. Here, we identified a pepper (Capsicum annuum) patatin-like phospholipase (CaPLP1) gene that is transcriptionally induced in pepper leaves by avirulent Xanthomonas campestris pv. vesicatoria (Xcv) infection. CaPLP1 containing an N-terminal signal peptide localized to the cytoplasm and plasma membrane, leading to the secretion into the apoplastic regions. Silencing of CaPLP1 in pepper conferred enhanced susceptibility to Xcv infection. Defense responses to Xcv, including the generation of reactive oxygen species (ROS), hypersensitive cell death and the expression of the salicylic acid (SA)-dependent marker gene CaPR1, were compromised in the CaPLP1-silenced pepper plants. Transient expression of CaPLP1 in pepper leaves induced the accumulation of fluorescent phenolics, expression of the defense marker genes CaPR1 and CaSAR82A, and generation of ROS, ultimately leading to the hypersensitive cell death response. Overexpression (OX) of CaPLP1 in Arabidopsis also conferred enhanced resistance to Pseudomonas syringae pv. tomato (Pst) and Hyaloperonospora arabidopsidis infection. CaPLP1-OX leaves showed reduced Pst growth, enhanced ROS burst and electrolyte leakage, induction of the defense response genes AtPR1, AtRbohD and AtGST, as well as constitutive activation of both the SA-dependent gene AtPR1 and the JA-dependent gene AtPDF1.2. Together, these results suggest that CaPLP1 is involved in plant defense and cell death signaling in response to microbial pathogens.
- Subjects :
- Agrobacterium
Plant Science
Biology
Phospholipase
Xanthomonas campestris
Pepper
Botany
Genetics
Pseudomonas syringae
Plant defense against herbivory
Gene Silencing
Phylogeny
Hyaloperonospora arabidopsidis
Cell Death
fungi
food and beverages
Xanthomonas campestris pv. Vesicatoria
General Medicine
Plants, Genetically Modified
biology.organism_classification
Cell biology
Patatin-like phospholipase
Phospholipases
Signal transduction
Capsicum
Agronomy and Crop Science
Signal Transduction
Subcellular Fractions
Subjects
Details
- ISSN :
- 15735028 and 01674412
- Volume :
- 84
- Database :
- OpenAIRE
- Journal :
- Plant Molecular Biology
- Accession number :
- edsair.doi.dedup.....a5c1c5cabaa7542de8541bf10dd184f2