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Expression and promoter analysis of mouse mastrin gene
- Source :
- Biochemical and biophysical research communications. 307(3)
- Publication Year :
- 2003
-
Abstract
- Human astrin is a newly identified microtubule-associated protein, which is highly expressed in the testis. Silencing of astrin has resulted in growth arrest and apoptotic cell death. In this study, we describe the cloning and genomic structure of mastrin, the mouse counterpart to astrin. The overall mouse mastrin amino-acid sequence is 66% identical to human astrin. Mastrin protein was demonstrated to localize to mitotic spindles during mitosis. Genomic clones containing mastrin gene were isolated; the gene was found to have 24 exons spanning 24kb of genomic DNA. Deletion analysis of 5(')-flanking sequences demonstrated that the first 120bp proximal to the TATA-less promoter region is necessary for minimal transcription of the mouse mastrin gene.
- Subjects :
- Transcriptional Activation
Transcription, Genetic
5' Flanking Region
5' flanking region
Molecular Sequence Data
Biophysics
Cell Cycle Proteins
Biology
Biochemistry
Exon
Mice
Transcription (biology)
Gene silencing
Animals
Humans
Tissue Distribution
Amino Acid Sequence
Cloning, Molecular
Promoter Regions, Genetic
Molecular Biology
Gene
Mitosis
Base Sequence
Promoter
Cell Biology
3T3 Cells
Molecular biology
genomic DNA
Microtubule-Associated Proteins
Sequence Alignment
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 307
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....a5adb7d6ccdd8ec8a4fbeb79576990e3