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General Study and Gene Expression Profiling of Endotheliocytes Cultivated on Electrospun Materials
- Source :
- Materials, Volume 12, Issue 24
- Publication Year :
- 2019
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2019.
-
Abstract
- Endothelization of the luminal surface of vascular grafts is required for their long-term functioning. Here, we have cultivated human endothelial cells (HUVEC) on different 3D matrices to assess cell proliferation, gene expression and select the best substrate for endothelization. 3D matrices were produced by electrospinning from solutions of poly(D,L-lactide-co-glycolide) (PLGA), polycaprolactone (PCL), and blends of PCL with gelatin (Gl) in hexafluoroisopropanol. Structure and surface properties of 3D matrices were characterized by SEM, AFM, and sessile drop analysis. Cell adhesion, viability, and proliferation were studied by SEM, Alamar Blue staining, and 5-ethynyl-2&rsquo<br />deoxyuridine (EdU) assay. Gene expression profiling was done on an Illumina HiSeq 2500 platform. Obtained data indicated that 3D matrices produced from PCL with Gl and treated with glutaraldehyde provide the most suitable support for HUVEC adhesion and proliferation. Transcriptome sequencing has demonstrated a minimal difference of gene expression profile in HUVEC cultivated on the surface of these matrices as compared to tissue culture plastic, thus confirming these matrices as the best support for endothelization.
- Subjects :
- food.ingredient
02 engineering and technology
macromolecular substances
Gelatin
Article
03 medical and health sciences
chemistry.chemical_compound
Tissue culture
food
polycaprolactone
Gene expression
General Materials Science
Cell adhesion
3D matrices
electrospinning
030304 developmental biology
0303 health sciences
technology, industry, and agriculture
RNA sequencing
Adhesion
021001 nanoscience & nanotechnology
Molecular biology
endothelization
Gene expression profiling
PLGA
chemistry
Glutaraldehyde
0210 nano-technology
vascular grafts
Subjects
Details
- Language :
- English
- ISSN :
- 19961944
- Database :
- OpenAIRE
- Journal :
- Materials
- Accession number :
- edsair.doi.dedup.....a5a7c32eaa7aef9b21e2497d6082c0f0
- Full Text :
- https://doi.org/10.3390/ma12244082