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Extracellular microRNA signature in chronic kidney disease
- Publication Year :
- 2017
- Publisher :
- American Physiological Society, 2017.
-
Abstract
- MicroRNAs (miRNAs) are noncoding RNAs that regulate posttranscriptional gene expression. In this study we characterized the circulating and urinary miRNA pattern associated with reduced glomerular filtration rate, using Affymetrix GeneChip miR 4.0 in 28 patients with chronic kidney disease (CKD). Top miRNA discoveries from the human studies were validated in an Alb/TGFβ mouse model of CKD, and in rat renal proximal tubular cells (NRK52E) exposed to TGFβ1. Plasma and urinary levels of procollagen III N-terminal propeptide and collagen IV were elevated in patients with decreased estimated glomerular filtration rate (eGFR). Expression of 384 urinary and 266 circulatory miRNAs were significantly different between CKD patients with eGFR ≥30 vs. −1·1.73 m−2. Pathway analysis mapped multiple miRNAs to TGFβ signaling-related mRNA targets. Specifically, Let-7a was significantly downregulated, and miR-130a was significantly upregulated, in urine of patients with eGFR
- Subjects :
- 0301 basic medicine
Adult
Genetic Markers
Male
Physiology
Mice, Transgenic
Biology
Bioinformatics
Kidney
Cell Line
Transcriptome
Transforming Growth Factor beta1
03 medical and health sciences
Transforming Growth Factor beta
Albumins
Gene expression
microRNA
medicine
Extracellular
Animals
Humans
Gene Regulatory Networks
Genetic Predisposition to Disease
Renal Insufficiency, Chronic
Aged
Oligonucleotide Array Sequence Analysis
Gene Expression Profiling
Transforming growth factor beta
Middle Aged
medicine.disease
Fibrosis
Rats
Gene expression profiling
Mice, Inbred C57BL
Disease Models, Animal
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
Phenotype
Cancer research
biology.protein
Mice, Inbred CBA
Female
Kidney disease
Research Article
Glomerular Filtration Rate
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....a5653f0d6fbf3a009c206b1f12bcab4d