Back to Search
Start Over
Control of seed dormancy in Arabidopsis by a cis -acting noncoding antisense transcript
- Source :
- Proceedings of the National Academy of Sciences. 113
- Publication Year :
- 2016
- Publisher :
- Proceedings of the National Academy of Sciences, 2016.
-
Abstract
- Seed dormancy is one of the most crucial process transitions in a plant's life cycle. Its timing is tightly controlled by the expression level of the Delay of Germination 1 gene (DOG1). DOG1 is the major quantitative trait locus for seed dormancy in Arabidopsis and has been shown to control dormancy in many other plant species. This is reflected by the evolutionary conservation of the functional short alternatively polyadenylated form of the DOG1 mRNA. Notably, the 3' region of DOG1, including the last exon that is not included in this transcript isoform, shows a high level of conservation at the DNA level, but the encoded polypeptide is poorly conserved. Here, we demonstrate that this region of DOG1 contains a promoter for the transcription of a noncoding antisense RNA, asDOG1, that is 5' capped, polyadenylated, and relatively stable. This promoter is autonomous and asDOG1 has an expression profile that is different from known DOG1 transcripts. Using several approaches we show that asDOG1 strongly suppresses DOG1 expression during seed maturation in cis, but is unable to do so in trans Therefore, the negative regulation of seed dormancy by asDOG1 in cis results in allele-specific suppression of DOG1 expression and promotes germination. Given the evolutionary conservation of the asDOG1 promoter, we propose that this cis-constrained noncoding RNA-mediated mechanism limiting the duration of seed dormancy functions across the Brassicaceae.
- Subjects :
- 0301 basic medicine
RNA, Untranslated
Transcription, Genetic
Polyadenylation
Arabidopsis
Biology
03 medical and health sciences
Gene Expression Regulation, Plant
Transcription (biology)
RNA, Antisense
Promoter Regions, Genetic
Gene
Conserved Sequence
Genetics
Multidisciplinary
Base Sequence
Arabidopsis Proteins
Seed dormancy
Exons
Plant Dormancy
biology.organism_classification
Antisense RNA
030104 developmental biology
PNAS Plus
RNA, Plant
Seeds
Dormancy
Trans-acting
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 113
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....a550733cef92a5ea765e4bd133b74bc6