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Effects of antibiotic treatment on microbiota, viral transmission and viral pathogenesis of MoMuLV ts1 infected BALB/c mice
- Source :
- PLoS ONE, Vol 17, Iss 1, p e0261689 (2022), PLoS ONE, PLoS ONE, Vol 17, Iss 1 (2022)
- Publication Year :
- 2022
- Publisher :
- Public Library of Science (PLoS), 2022.
-
Abstract
- The effects of normal and altered intestinal microbiota on murine retroviral transmission via the gastrointestinal tract (GIT) are diverse. The role of orally administered antibiotic treatment (ABX) on viral transmission, GIT microbial dysbiosis and subsequent pathogenesis of Moloney Murine Leukemia virus–temperature sensitive 1 (ts1) on BALB/c mice were studied. BALB/c mice were divided into four groups: ABXts1—Treatment/Infection; ABX—Treatment/No infection; ts1—No treatment/Infection; Ctrl (control)—No treatment/No infection. ABXts1 and ABX groups showed a significant phylogenetic shift (ANOSIM p-value = 0.001) in alpha and beta diversity comparisons for microbial community composition compared to Ctrl group. Mice in the ABXts1 and ABX groups showed megacolon compared to ts1 and Ctrl groups; ABXts1 and ts1 groups showed hepatosplenomegaly, thymus enlargement, and mesenteric lymphadenopathy compared to ABX and Ctrl groups. Ctrl group had no abnormal manifestations. ABX treatment and ts1 infection uniquely affect microbial community when compared to control: ABXts1 and ABX groups significantly reduce microbiome diversity by over 80% and ts1 group by over 30%. ABXts1 and ts1 groups’ viral load and clinical manifestations of infection were comparable; antibiotic treatment did not notably affect ts1 infection. Transmission and pathophysiology of ts1 infection were not significantly altered by the microbial composition of the GI tract, but ts1 viral infection did result in microbial dysbiosis independent of antibiotic treatment.
- Subjects :
- Physiology
Science
Immunology
Mouse Models
Microbial Genomics
Research and Analysis Methods
Microbiology
Mice
Model Organisms
Antibiotics
Microbial Control
Virology
Immune Physiology
Medicine and Health Sciences
Genetics
Animals
Pharmacology
Mice, Inbred BALB C
Multidisciplinary
Bacteria
Antimicrobials
Gut Bacteria
Body Weight
Organisms
Drugs
Biology and Life Sciences
Genomics
Animal Models
Anti-Bacterial Agents
Gastrointestinal Microbiome
Thymus
Physiological Parameters
Experimental Organism Systems
Medical Microbiology
Immune System
Animal Studies
Medicine
Female
Microbiome
Moloney murine leukemia virus
Viral Transmission and Infection
Spleen
Retroviridae Infections
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 17
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....a54d11449215148e7290caa6a210a053