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Immune repertoire in the transcriptome of Littorina littorea reveals new trends in lophotrochozoan proto-complement evolution
- Source :
- Developmental & Comparative Immunology. 84:250-263
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- The evolution of complement system in invertebrates is poorly investigated. While the repertoire of complement genes in several Ecdysozoa lineages is found substantially different from that of Deuterostomia, the composition and function of the complement in the second protostome lineage, Lophotrochozoa, remains unclear. Here we report the general description of new transcriptomic data on the common periwinkle, Littorina littorea, and trace the evolutionary trajectories of the ancestral proto-complement repertoire. The repertoire is defined as immune cascade providing the minimum set of C3-associated molecules required for C3b amplification, opsonization of the targets and their phagocytosis: thioester protein (TEP) C3, serine protease C2/factor B (Bf) and complement receptors (CR). The reference transcriptome of L. littorea was built from the dual-species RNA-seq experiment with the periwinkle and its tissue digenean parasite Himasthla elongata. Five TEPs, including the ortholog of the C3, are found expressed in the in the mollusk's inflamed tissues. The homolog of the complement receptors CR1/CR2 is also expressed, however the ortholog of Bf is not. The extensive phylogenetic analysis showed that the C3 ortholog and the complement receptors are retained in all key lophotrochozoan taxa: Mollusca, Annelida and Brachiopoda. However, the Bf ortholog was lost at least three times independently in different lineages: i) Cephalopoda, ii) a common ancestor of all Gastropoda and iii) one of the Annelida lineage, Clitellata. Both C3 and Bf molecules were retained in bivalve species, brachiopods and annelid worms from the Polychaeta lineage. Hypothetically, the function of the lost Bf in these animals can be compensated by Factor L (Lf) – the serine protease first found in L. littorea and homologous to both, the Bf and the arthropod factor C (Cf). The contrast differences in proto-complement repertoire between the sister mollusk’ taxa, Bivalvia and Gastropoda (the conserved and modified sets, respectively), can underlie differences in their susceptibility to digenean infection.
- Subjects :
- 0301 basic medicine
Annelida
Lineage (evolution)
Gastropoda
Immunology
Lophotrochozoa
Trematode Infections
Complement receptor
Complement factor B
Host-Parasite Interactions
03 medical and health sciences
Phagocytosis
Animals
Humans
Complement Activation
Phylogeny
Inflammation
Phylogenetic tree
biology
Gene Expression Profiling
biology.organism_classification
Biological Evolution
Invertebrates
Immunity, Innate
Receptors, Complement
Complement system
030104 developmental biology
Cephalopoda
Evolutionary biology
Complement C3b
Protostome
Trematoda
Transcriptome
Ecdysozoa
Complement Factor B
Developmental Biology
Subjects
Details
- ISSN :
- 0145305X
- Volume :
- 84
- Database :
- OpenAIRE
- Journal :
- Developmental & Comparative Immunology
- Accession number :
- edsair.doi.dedup.....a5439b296af7a9652a616e705f92bf0d