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A paper-based calorimetric microfluidics platform for bio-chemical sensing
- Source :
- Biosensorsbioelectronics. 59
- Publication Year :
- 2013
-
Abstract
- In this report, a paper-based micro-calorimetric biochemical detection method is presented. Calorimetric detection of biochemical reactions is demonstrated as an extension of current colorimetric and electrochemical detection mechanisms of paper-based biochemical analytical systems. Reaction and/or binding temperature of glucose/glucose oxidase, DNA/hydrogen peroxide, and biotin/streptavidin, are measured by the paper-based micro-calorimeter. Commercially available glucose calibration samples of 0.05, 0.15 and 0.3% wt/vol concentration are used for comparing the device performance with a commercially available glucose meter (electrochemical detection). The calorimetric glucose detection demonstrates a measurement error less than 2%. The calorimetric detection results of DNA concentrations from 0.9 to 7.3 mg/mL and temperature changes in biotin and streptavidin reaction are presented to demonstrate the feasibility of integrating the calorimetric detection method with paper based microfluidic devices.
- Subjects :
- Streptavidin
Paper
Microfluidics
Biomedical Engineering
Biophysics
Analytical chemistry
Biosensing Techniques
Calorimetry
Biochemical detection
chemistry.chemical_compound
Biotin
Limit of Detection
Electrochemistry
Glucose oxidase
Hydrogen peroxide
Chromatography
biology
Glucose meter
General Medicine
Paper based
DNA
Equipment Design
Microfluidic Analytical Techniques
Glucose
chemistry
Calibration
biology.protein
Colorimetry
Biotechnology
Subjects
Details
- ISSN :
- 18734235
- Volume :
- 59
- Database :
- OpenAIRE
- Journal :
- Biosensorsbioelectronics
- Accession number :
- edsair.doi.dedup.....a5438b052cc740caf96b185a3a92b63b