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Neonatal Immunization with a Single IL-4/Antigen Dose Induces Increased Antibody Responses after Challenge Infection with Equine Herpesvirus Type 1 (EHV-1) at Weanling Age
- Source :
- PLoS ONE, Vol 12, Iss 1, p e0169072 (2017), PLoS ONE
- Publication Year :
- 2017
- Publisher :
- Public Library of Science (PLoS), 2017.
-
Abstract
- Neonatal foals respond poorly to conventional vaccines. These vaccines typically target T-helper (Th) cell dependent B-cell activation. However, Th2-cell immunity is impaired in foals during the first three months of life. In contrast, neonatal basophils are potent interleukin-4 (IL-4) producers. The purpose of this study was to develop a novel vaccine triggering the natural capacity of neonatal basophils to secrete IL-4 and to evaluate if vaccination resulted in B-cell activation and antibody production against EHV-1 glycoprotein C (gC). Neonatal vaccination was performed by oral biotinylated IgE (IgE-bio) treatment at birth followed by intramuscular injection of a single dose of streptavidin-conjugated gC/IL-4 fusion protein (Sav-gC/IL-4) for crosslinking of receptor-bound IgE-bio (group 1). Neonates in group 2 received the intramuscular Sav-gC/IL-4 vaccine only. Group 3 remained non-vaccinated at birth. After vaccination, gC antibody production was not detectable. The ability of the vaccine to induce protection was evaluated by an EHV-1 challenge infection after weaning at 7 months of age. Groups 1 and 2 responded to EHV-1 infection with an earlier onset and overall significantly increased anti-gC serum antibody responses compared to control group 3. In addition, group 1 weanlings had a decreased initial fever peak after infection indicating partial protection from EHV-1 infection. This suggested that the neonatal vaccination induced a memory B-cell response at birth that was recalled at weanling age after EHV-1 challenge. In conclusion, early stimulation of neonatal immunity via the innate arm of the immune system can induce partial protection and increased antibody responses against EHV-1.<br />Funding for this project was provided by the Harry M. Zweig Memorial Fund for Equine Research at Cornell University ‘A Novel Strategy to Boost Antibody Production to EHV-1 in Neonates’ (http://vet.cornell.edu/research/Zweig/). Monoclonal antibody development for horse cell surface markers and cytokines was supported by USDA grant #2005-01812 ‘The US Veterinary Immune Reagent Network’ and #2015-67015-23072 ‘Equine Immune Reagents: Development of monoclonal antibodies to improve the analysis of immunity in horses’ (https://nifa.usda.gov/).
- Subjects :
- 0301 basic medicine
Hestar
B Cells
Physiology
lcsh:Medicine
Antibodies, Viral
Lymphocyte Activation
Immunoglobulin E
Biochemistry
White Blood Cells
Viral Envelope Proteins
Animal Cells
Immune Physiology
Medicine and Health Sciences
Public and Occupational Health
lcsh:Science
Mammals
B-Lymphocytes
Innate Immune System
Vaccines
Immune System Proteins
Multidisciplinary
T Cells
Temperature
Herpesviridae Infections
Vaccination and Immunization
Frumur
Vaccination
Vertebrates
Nýburar
Cytokines
Cellular Types
Antibody
Intramuscular injection
Herpesvirus 1, Equid
Research Article
Recombinant Fusion Proteins
Immune Cells
Immunology
Equines
Weanling
Herpesvirus Vaccines
Biology
Antibodies
03 medical and health sciences
Immune system
Antigen
Neutralization Tests
Immunity
Animals
Horses
Antibody-Producing Cells
Secretion
Blood Cells
lcsh:R
Organisms
Biology and Life Sciences
Proteins
Neonates
Cell Biology
Molecular Development
Bóluefni
Mótefni
030104 developmental biology
Animals, Newborn
Immune System
Antibody Formation
Amniotes
biology.protein
Horse Diseases
lcsh:Q
Interleukin-4
Preventive Medicine
Physiological Processes
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....a51e68c5a4e5c01629a1fc28e2b63a8e