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Characterization of discrete subpopulations of progenitor cells in traumatic human extremity wounds
- Source :
- PLoS ONE, PLoS ONE, Vol 9, Iss 12, p e114318 (2014)
- Publication Year :
- 2014
-
Abstract
- Here we show that distinct subpopulations of cells exist within traumatic human extremity wounds, each having the ability to differentiate into multiple cells types in vitro. A crude cell suspension derived from traumatized muscle was positively sorted for CD29, CD31, CD34, CD56 or CD91. The cell suspension was also simultaneously negatively sorted for either CD45 or CD117 to exclude hematopoietic stem cells. These subpopulations varied in terms their total numbers and their abilities to grow, migrate, differentiate and secrete cytokines. While all five subpopulations demonstrated equal abilities to undergo osteogenesis, they were distinct in their ability to undergo adipogenesis and vascular endotheliogenesis. The most abundant subpopulations were CD29+ and CD34+, which overlapped significantly. The CD29+ and CD34+ cells had the greatest proliferative and migratory capacity while the CD56+ subpopulation produced the highest amounts of TGFß1 and TGFß2. When cultured under endothelial differentiation conditions the CD29+ and CD34+ cells expressed VE-cadherin, Tie2 and CD31, all markers of endothelial cells. These data indicate that while there are multiple cell types within traumatized muscle that have osteogenic differentiation capacity and may contribute to bone formation in post-traumatic heterotopic ossification (HO), the major contributory cell types are CD29+ and CD34+, which demonstrate endothelial progenitor cell characteristics.
- Subjects :
- Physiology
Cell Transplantation
Cellular differentiation
CD34
Cell Movement
Osteogenesis
Animal Cells
Medicine and Health Sciences
Stem Cell Niche
Multidisciplinary
Adipogenesis
Stem Cells
Stem Cell Therapy
Cell Differentiation
Flow Cytometry
Cell biology
Endothelial stem cell
Haematopoiesis
Adult Stem Cells
Lower Extremity
Medicine
Engineering and Technology
Stem cell
Cellular Types
Research Article
Biotechnology
Cell type
Science
Bioengineering
Surgical and Invasive Medical Procedures
Biology
Endothelial progenitor cell
Tissue Repair
Humans
Progenitor cell
Cell Proliferation
Clinical Genetics
Wound Healing
Transplantation
Tissue Engineering
Ossification, Heterotopic
Endothelial Cells
Biology and Life Sciences
Extremities
Mesenchymal Stem Cells
Cell Biology
Immunology
Physiological Processes
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....a50d62642decd54108b28d0d14754117