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Identification of Myocardial and Vascular Precursor Cells in Human and Mouse Epicardium
- Source :
- Circulation Research. 101:1255-1265
- Publication Year :
- 2007
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2007.
-
Abstract
- During cardiac development, the epicardium is the source of multipotent mesenchymal cells, which give rise to endothelial and smooth muscle cells in coronary vessels and also, possibly, to cardiomyocytes. The aim of the present study was to determine whether stem cells are retained in the adult human and murine epicardium and to investigate the regenerative potential of these cells following acute myocardial infarction. We show that c-kit + and CD34 + cells can indeed be detected in human fetal and adult epicardium and that they represent 2 distinct populations. Both subsets of cells were negative for CD45, a cell surface marker that identifies the hematopoietic cell lineage. Immunofluorescence revealed that freshly isolated c-kit + and CD34 + cells expressed early and late cardiac transcription factors and could acquire an endothelial phenotype in vitro. In the murine model of myocardial infarction, there was an increase in the absolute number and proliferation of epicardial c-kit + cells 3 days after coronary ligation; at this time point, epicardial c-kit + cells were identified in the subepicardial space and expressed GATA4. Furthermore, 1 week after myocardial infarction, cells coexpressing c-kit + , together with endothelial or smooth muscle cell markers, were identified in the wall of subepicardial blood vessels. In summary, the postnatal epicardium contains a cell population with stem cell characteristics that retains the ability to give rise to myocardial precursors and vascular cells. These cells may play a role in the regenerative response to cardiac damage.
- Subjects :
- Pathology
medicine.medical_specialty
Physiology
Population
Cell
CD34
Biology
Stem cell marker
Mice
Fetal Heart
Cell Movement
Precursor cell
medicine
Animals
Humans
Myocytes, Cardiac
education
education.field_of_study
Myocardium
Stem Cells
Mesenchymal stem cell
Mice, Inbred C57BL
Endothelial stem cell
medicine.anatomical_structure
cardiovascular system
Female
Endothelium, Vascular
Stem cell
Cardiology and Cardiovascular Medicine
Pericardium
Subjects
Details
- ISSN :
- 15244571 and 00097330
- Volume :
- 101
- Database :
- OpenAIRE
- Journal :
- Circulation Research
- Accession number :
- edsair.doi.dedup.....a50acbbd169f61efb9bf22c7f1cc253f