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New staining method using methionyl-tRNA synthetase 1 antibody for brushing cytology of bile duct cancer
- Source :
- Gastrointestinal endoscopy. 92(2)
- Publication Year :
- 2019
-
Abstract
- Identifying malignant biliary strictures using endobiliary brushing cytology specimens is important for treatment decision-making and prognosis prediction. The sensitivity of brushing cytology specimens based on Papanicolaou (Pap) staining is low, which hampers accurate diagnosis of indeterminate strictures. Here, we assessed the diagnostic value of immunohistochemical (IHC) and immunofluorescence (IF) staining for methionyl-tRNA synthetase 1 (MARS1).Endobiliary brushing cytology specimens were obtained during ERCP from 80 patients with an extrahepatic biliary stricture. Pap and MARS1 IF staining were performed on liquid-based cytology slides derived from these specimens. Sections of bile duct adenocarcinoma and normal bile duct tissue were obtained from 45 patients who underwent surgery for malignant biliary stricture, and MARS1 levels were evaluated by IHC staining.MARS1 IF staining was applied to brushing cytology specimens, and the results showed strong signals in malignant biliary structures but not in the negative for malignancy specimens. Sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were 70.4%, 96.2%, 97.4%, 56.8%, and 78.8%, respectively, for conventional Pap staining and 98.1%, 96.1%, 98.1%, 96.2%, and 97.5%, respectively, for MARS1 IF (P .0001). When IHC staining was used, MARS1 was detected in 45 bile duct adenocarcinoma sections but not in 15 normal bile duct sections. Moreover, MARS1 mRNA and protein levels were significantly higher in bile duct adenocarcinoma sections according to polymerase chain reaction and Western blot, respectively.The high sensitivity and accuracy of MARS1 IF staining enabled detection of malignancy in patients with indeterminate biliary stricture. Further prospective studies are needed to validate our findings. (Clinical trial registration number: KCT 0003285.).
- Subjects :
- medicine.medical_specialty
Papanicolaou stain
Methionine-tRNA Ligase
Malignancy
Gastroenterology
Sensitivity and Specificity
Bile duct cancer
03 medical and health sciences
0302 clinical medicine
Internal medicine
Cytology
medicine
Humans
Radiology, Nuclear Medicine and imaging
Prospective Studies
Prospective cohort study
Bile Duct Adenocarcinoma
Cholangiopancreatography, Endoscopic Retrograde
Staining and Labeling
business.industry
medicine.disease
Staining
Bile Ducts, Intrahepatic
Bile Duct Neoplasms
030220 oncology & carcinogenesis
Immunohistochemistry
030211 gastroenterology & hepatology
Bile Ducts
business
Subjects
Details
- ISSN :
- 10976779
- Volume :
- 92
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Gastrointestinal endoscopy
- Accession number :
- edsair.doi.dedup.....a4bd50b3f10fc23f8c31c878f5303f5a