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Photodynamic killing of human squamous cell carcinoma cells using a monoclonal antibody-photosensitizer conjugate
- Source :
- Journal of the National Cancer Institute. 83(17)
- Publication Year :
- 1991
-
Abstract
- We have developed procedures in which the photosensitizer benzoporphyrin derivative monoacid ring A (BPD) can be covalently linked to carrier molecules of modified polyvinyl alcohol (PVA) to produce water-soluble PVA-BPD conjugates with a molecular mass in the range of 30 kd. These carriers can subsequently be covalently linked to monoclonal antibodies (MoAbs) using heterobifunctional linking agents. We describe here such a conjugate in which the MoAb (5E8) has specificity for a glycoprotein detected on human squamous cell carcinomas of the lung. We provide evidence that the conjugates produced were covalently linked and retained both their photosensitizing and antigen-binding activities. We show further that the MoAb-PVA-BPD conjugate, in the presence of 10% fetal calf serum, exhibited highly enhanced phototoxic killing of the target cell line (A549) over that exhibited by free BPD or a control MoAb-PVA-BPD conjugate. These results demonstrate, therefore, both the selectivity and specificity of this MoAb conjugate.
- Subjects :
- Cancer Research
Radiation-Sensitizing Agents
Porphyrins
medicine.drug_class
Cell Survival
Enzyme-Linked Immunosorbent Assay
Monoclonal antibody
Sensitivity and Specificity
mental disorders
medicine
Tumor Cells, Cultured
Humans
Photosensitizer
Cytotoxicity
chemistry.chemical_classification
integumentary system
Molecular mass
biology
Molecular Structure
Antibodies, Monoclonal
In vitro
Oncology
Biochemistry
chemistry
Photochemotherapy
Polyvinyl Alcohol
biology.protein
Carcinoma, Squamous Cell
Electrophoresis, Polyacrylamide Gel
Antibody
Glycoprotein
Conjugate
Subjects
Details
- ISSN :
- 00278874
- Volume :
- 83
- Issue :
- 17
- Database :
- OpenAIRE
- Journal :
- Journal of the National Cancer Institute
- Accession number :
- edsair.doi.dedup.....a49c464051e2b8961a636c40decbfca9