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The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes
- Source :
- The EMBO Journal. 22:1370-1380
- Publication Year :
- 2003
- Publisher :
- Wiley, 2003.
-
Abstract
- Recent reports have increased our knowledge of the consecutive steps during 60S ribosome biogenesis substantially, but 40S subunit formation is less well understood. Here, we investigate the maturation of nucleolar 90S pre-ribosomes into cytoplasmic 40S pre-ribosomes. During the transition from 90S to 40S particles, the majority of non-ribosomal proteins (approximately 30 species) dissociate, and significantly fewer factors associate with 40S pre-ribosomes. Notably, some of these components are part of both early 90S and intermediate 40S pre-particles in the nucleolus (e.g. Enp1p, Dim1p and Rrp12p), whereas others (e.g. Rio2p and Nob1p) are found mainly on late cytoplasmic pre-40S subunits. Finally, temperature-sensitive mutants mapping either in earlier (enp1-1) or later (rio2-1) components exhibit defects in the formation and nuclear export of pre-40S subunits. Our data provide an initial biochemical map of the pre-40S ribosomal subunit on its path from the nucleolus to the cytoplasm. This pathway involves fewer changes in composition than seen during 60S biogenesis.
- Subjects :
- Ribosomal Proteins
Cytoplasm
Saccharomyces cerevisiae Proteins
Nucleolus
Protein subunit
Green Fluorescent Proteins
Ribosome biogenesis
Biology
Models, Biological
Ribosome
General Biochemistry, Genetics and Molecular Biology
Ribosomal protein
Gene Expression Regulation, Fungal
RNA Precursors
Eukaryotic Small Ribosomal Subunit
RNA Processing, Post-Transcriptional
Molecular Biology
Heat-Shock Proteins
General Immunology and Microbiology
Eukaryotic Large Ribosomal Subunit
General Neuroscience
Biological Transport
RNA, Fungal
Articles
Cell biology
Luminescent Proteins
Mutation
Ribosomes
Cell Nucleolus
Subjects
Details
- ISSN :
- 14602075
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- The EMBO Journal
- Accession number :
- edsair.doi.dedup.....a48ea32ab76069d068e4e3add4833610