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Intestinal dysbiosis in pediatric Crohn's disease patients with IL10RA mutations
- Source :
- World Journal of Gastroenterology
- Publication Year :
- 2020
- Publisher :
- Baishideng Publishing Group Inc, 2020.
-
Abstract
- Background Several studies have employed animal models to explore the association between microbiota and interleukin (IL) 10 signaling; however, limited information is available about the human microbiome. Aim To characterize the microbiome in patients with IL10RA mutations and to explore the association between gut dysbiosis and disease severity. Methods Fecal samples were collected from patients who were diagnosed with loss-of-function mutations in the IL10RA gene between January 2017 and July 2018 at the Children's Hospital of Fudan University. Age-matched volunteer children were recruited as healthy controls. Patients with Crohn's disease (CD) were used as disease controls to standardize the antibiotic exposure. Microbial DNA was extracted from the fecal samples. All analyses were based on the 16S rRNA gene sequencing data. Results Seventeen patients with IL10RA mutations (IL10RA group), 17 patients with pediatric CD, and 26 healthy children were included. Both patients with IL10RA mutations and those with CD exhibited a reduced diversity of gut microbiome with increased variability. The relative abundance of Firmicutes was substantially increased in the IL10RA group (P = 0.02). On further comparison of the relative abundance of taxa between patients with IL10RA mutations and healthy children, 13 taxa showed significant differences. The IL10RA-specific dysbiosis indices exhibited a significant positive correlation with weighted pediatric CD activity index and simple endoscopic score for CD. Conclusion In patients with IL10RA mutations and early onset inflammatory bowel disease, gut dysbiosis shows a moderate association with disease severity.
- Subjects :
- medicine.medical_specialty
Interleukin-10 Receptor alpha Subunit
Observational Study
Disease
Gut microbiota
Gut flora
Gastroenterology
Inflammatory bowel disease
03 medical and health sciences
Feces
0302 clinical medicine
IL10RA gene
Crohn Disease
Internal medicine
RNA, Ribosomal, 16S
medicine
Humans
Microbiome
Child
Disease severity
Pediatric
Crohn's disease
biology
business.industry
Human microbiome
General Medicine
biology.organism_classification
medicine.disease
030220 oncology & carcinogenesis
Mutation
Dysbiosis
030211 gastroenterology & hepatology
business
Subjects
Details
- Language :
- English
- ISSN :
- 22192840 and 10079327
- Volume :
- 26
- Issue :
- 22
- Database :
- OpenAIRE
- Journal :
- World Journal of Gastroenterology
- Accession number :
- edsair.doi.dedup.....a48d214dc02fc4e1025bf9d61a5bb50e