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Interlaboratory trial of the rat Pig-a mutation assay using an erythroid marker HIS49 antibody
- Source :
- Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 755:126-134
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- The peripheral blood Pig-a assay has shown promise as a tool for evaluating in vivo mutagenicity. In this study five laboratories participated in a collaborative trial that evaluated the transferability and reproducibility of a rat Pig-a assay that uses a HIS49 antibody reacts with an antigen found on erythrocytes and erythroid progenitors. In preliminary work, flow cytometry methods were established that enabled all laboratories to detect CD59-negative erythrocyte frequencies (Pig-a mutant frequencies) of
- Subjects :
- Male
Erythrocytes
Reticulocytes
Glycosylphosphatidylinositols
9,10-Dimethyl-1,2-benzanthracene
Health, Toxicology and Mutagenesis
DMBA
CD59 Antigens
Gene mutation
medicine.disease_cause
Sensitivity and Specificity
Flow cytometry
Japan
Antigen
In vivo
Genetics
medicine
Animals
Erythroid Precursor Cells
Mutation
biology
medicine.diagnostic_test
Mutagenicity Tests
Erythrocyte Membrane
Antibodies, Monoclonal
Membrane Proteins
Reproducibility of Results
Flow Cytometry
Molecular biology
4-Nitroquinoline-1-oxide
Peripheral blood
Rats
Ethylnitrosourea
biology.protein
Antibody
Laboratories
Subjects
Details
- ISSN :
- 13835718
- Volume :
- 755
- Database :
- OpenAIRE
- Journal :
- Mutation Research/Genetic Toxicology and Environmental Mutagenesis
- Accession number :
- edsair.doi.dedup.....a48855723f6446efc0d1367c8838a58d