Characterization and in vitro and in vivo assessment of poly(butylene adipate-co-terephthalate)/nano-hydroxyapatite composites as scaffolds for bone tissue engineering

Made available in DSpace on 2019-10-04T11:56:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-02-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) National Council for Scientific and Technological Development The polyester for bone tissue engineering is produced using different concentrations of hydroxyapatite. Poly(butylene adipate-co-terephthalate) (PBAT) solutions containing different concentrations of nano-hydroxyapatite (nHAp) (1, 2, 3, 4, 5 and 6% wt) were evaluated to assess their potential to produce scaffolds via electrospinning. The characteristics of these solutions were evaluated using surface tension analysis. Different solutions were electrospun and characterized using scanning electron microscopy, X-ray diffractometry (XRD), infrared spectroscopy (FTIR), and differential scanning calorimetry analysis. In vitro and in vivo experiments were performed using MG-63 osteoblast cells and male Wistar rats. The MTT assay was used to evaluate the cytotoxicity of scaffolds. Microtomography and a three-point bend test were used to analyze parameters of bone neoformation 4 weeks after implantation. We observed a requirement for solutions containing nHAp to reach chemical stability to produce ultrathin fibers. FTIR and XRD data demonstrated the presence of carbonate and phosphate groups, and thermal analysis showed a reduction in crystallinity of the nanocomposites when the concentration of nHAp was increased. None of the scaffolds analyzed demonstrated cytotoxicity when compared to controls. All of the PBAT/nHAp scaffolds analyzed promoted bone repair; however, a solution of PBAT with 3% nHAp improved bone volume, force, and stiffness when compared to controls. Univ Porto, Fac Engn FEUP, P-4200465 Porto, Portugal Univ Brasil, Inst Cient & Tecnol, BR-08230030 Sao Paulo, SP, Brazil Ctr Univ UNINOVAFAPI, BR-64073505 Teresina, Piaui, Brazil Univ Estadual Paulista, Inst Ciencia & Tecnol, BR-12245000 Sao Jose Dos Campos, SP, Brazil Univ Fed Piaui, Lab Interdisciplinhar Mat Avancados, Programa Posgrad Ciencia & Engn Mat, Campus Univ Ministro Petronio Portella Bairro, BR-64049550 Teresina, Piaui, Brazil Univ Estadual Paulista, Inst Ciencia & Tecnol, BR-12245000 Sao Jose Dos Campos, SP, Brazil FAPESP: 2011/17877-7 FAPESP: 2015/01259-3 FAPESP: 2011/20345-7 FAPESP: 2016/04618-7 National Council for Scientific and Technological Development: 303752/2017-3 National Council for Scientific and Technological Development: 304133/2017-5