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Actin-based protrusions of migrating neutrophils are intrinsically lamellar and facilitate direction changes
- Source :
- eLife, Vol 6 (2017), eLife
- Publication Year :
- 2017
- Publisher :
- eLife Sciences Publications, Ltd, 2017.
-
Abstract
- Leukocytes and other amoeboid cells change shape as they move, forming highly dynamic, actin-filled pseudopods. Although we understand much about the architecture and dynamics of thin lamellipodia made by slow-moving cells on flat surfaces, conventional light microscopy lacks the spatial and temporal resolution required to track complex pseudopods of cells moving in three dimensions. We therefore employed lattice light sheet microscopy to perform three-dimensional, time-lapse imaging of neutrophil-like HL-60 cells crawling through collagen matrices. To analyze three-dimensional pseudopods we: (i) developed fluorescent probe combinations that distinguish cortical actin from dynamic, pseudopod-forming actin networks, and (ii) adapted molecular visualization tools from structural biology to render and analyze complex cell surfaces. Surprisingly, three-dimensional pseudopods turn out to be composed of thin (
- Subjects :
- amoeboid motility
0301 basic medicine
Materials science
cell migration
Neutrophils
QH301-705.5
1.1 Normal biological development and functioning
Science
Arp2/3 complex
HL-60 Cells
Lattice light-sheet microscopy
Time-Lapse Imaging
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Actin remodeling of neurons
Cell Movement
Underpinning research
cell biology
medicine
Humans
Pseudopodia
human
Biology (General)
visualization
Actin
Microscopy
General Immunology and Microbiology
biology
General Neuroscience
Actin remodeling
Cell Biology
General Medicine
Complex cell
Actins
Cell biology
030104 developmental biology
medicine.anatomical_structure
microscopy
arp2/3
biology.protein
Medicine
Biochemistry and Cell Biology
Lamellipodium
actin
Research Article
Human
Subjects
Details
- ISSN :
- 2050084X
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- eLife
- Accession number :
- edsair.doi.dedup.....a48087b51e47c3d4ae33a6a7fdb7982d