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The R2R3-MYB gene PsMYB58 positively regulates anthocyanin biosynthesis in tree peony flowers
- Source :
- Plant physiology and biochemistry : PPB. 164
- Publication Year :
- 2021
-
Abstract
- Tree peony (Paeonia suffruticosa Andr.) is a well-known ornamental flower in China with diverse colors. Flower color is one of the most important economic characteristics of tree peony and is mainly determined by anthocyanins. In this study, we cloned a PsMYB58 gene, which contained a 654 bp open reading frame (ORF), encoding a polypeptide of 218 amino acids. Sequence and phylogenetic analysis indicated that PsMYB58 is an anthocyanin regulatory R2R3-MYB gene. The transcription levels of PsMYB58 in different developmental stages of tree peony flowers were similar to those of the anthocyanin biosynthetic genes PsCHS, PsCHI, PsDFR, and PsANS. A bimolecular fluorescence complementation assay showed that PsMYB58 interacted with PsbHLH1 and PsbHLH3 in vivo. The overexpression of PsMYB58 in tobacco enhanced anthocyanin accumulation in various organs. Comparative transcriptome analysis showed that 943 genes were upregulated and 1203 downregulated in PsMYB58 transgenic tobacco, among which genes involved in the anthocyanin pathway were positively activated. Real-time quantitative PCR analysis verified that anthocyanin biosynthetic genes, including NtCHS, NtCHI, NtF3H, NtF3′H, NtDFR, and NtANS, and an anthocyanin regulatory bHLH gene, NtAN1b, were significantly upregulated in PsMYB58 transgenic tobacco. Our results indicated that PsMYB58 is a positive anthocyanin regulator in tree peony flowers. In summary, the functional identification of PsMYB58 furthers our understanding of the mechanism of peony flower color formation, thus providing a foundation for flower color improvement and molecular breeding.
- Subjects :
- 0106 biological sciences
0301 basic medicine
China
Physiology
Genes, myb
Plant Science
Flowers
Biology
Paeonia
01 natural sciences
Transcriptome
Anthocyanins
03 medical and health sciences
chemistry.chemical_compound
Transcription (biology)
Gene Expression Regulation, Plant
Genetics
MYB
Gene
Phylogeny
Plant Proteins
Phylogenetic tree
fungi
Paeonia suffruticosa
food and beverages
biology.organism_classification
Plants, Genetically Modified
Open reading frame
030104 developmental biology
chemistry
Anthocyanin
010606 plant biology & botany
Subjects
Details
- ISSN :
- 18732690
- Volume :
- 164
- Database :
- OpenAIRE
- Journal :
- Plant physiology and biochemistry : PPB
- Accession number :
- edsair.doi.dedup.....a4702bb889f19e79a4cddf3dd115528c