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Modulatory effect of aquaporin 5 on estrogen-induced epithelial-mesenchymal transition in prostate epithelial cells
- Source :
- Chinese Medical Journal, Vol 134, Iss 4, Pp 448-455 (2021), Chinese Medical Journal
- Publication Year :
- 2020
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2020.
-
Abstract
- Background. Estrogen is involved in the pathophysiological process of benign prostatic hyperplasia (BPH), in which epithelial-mesenchymal transition (EMT) plays an important role. Upregulation of aquaporin (AQP) 5, which is directly activated by estrogen, has been reported to promote EMT in multiple cells. This study aimed to examine the effects of AQP5 on estrogen-induced EMT in the prostate. Methods. Normal prostate (NP) tissue samples without any histopathological changes and BPH tissue samples with pathologically confirmed hyperplasia were obtained. An EMT cell model was subsequently established by adding estradiol (E2) to RWPE-1 cells, after which AQP5 knockdown was performed. Tissue morphological and immunohistochemical features were examined using hematoxylin-eosin and immunohistochemical staining. Western blot analysis was performed to determine the expression of AQPs, estrogen receptors, and EMT-related proteins. Cell proliferation was assessed and supernatants were collected for enzyme-linked immunosorbent assay to determine transforming growth factor-β1 (TGF-β1) concentrations. Immunofluorescence staining was performed to assess protein expressions in RWPE-1 cells. Results. BPH tissues exhibited greater EMT (TGF-β1: 1.362 ± 0.196 vs. 0.107 ± 0.067, P = 0.003; vimentin: 1.581 ± 0.508 vs. 0.221 ± 0.047, P
- Subjects :
- Male
Epithelial-Mesenchymal Transition
medicine.drug_class
lcsh:Medicine
Estrogen receptor
Vimentin
Transforming Growth Factor beta1
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Prostate
Humans
Medicine
Epithelial–mesenchymal transition
Benign prostatic hyperplasia
biology
business.industry
Aquaporin
lcsh:R
Epithelial Cells
Estrogens
Original Articles
General Medicine
Hyperplasia
Cadherins
medicine.disease
Estrogen
Aquaporin 5
medicine.anatomical_structure
030220 oncology & carcinogenesis
biology.protein
Cancer research
Immunohistochemistry
Prostate epithelial cells
business
030217 neurology & neurosurgery
Signal Transduction
Subjects
Details
- ISSN :
- 25425641 and 03666999
- Volume :
- 134
- Database :
- OpenAIRE
- Journal :
- Chinese Medical Journal
- Accession number :
- edsair.doi.dedup.....a469b40e2cfbd3d17862ecec1ed47a18