Determination of propranolol and its major metabolites in plasma and urine by high-performance liquid chromatography without solvent extraction

Fast, reliable, specific and sensitive methods are reported to accurately quantitate unchanged propranolol in plasma, and its major metabolites in plasma and urine after enzymatic hydrolysis without the need for solvent extraction. These methods enable the analyst to process a large number of propranolol samples in one working day and should prove valuable to clinical laboratories demanding both speed and specificity in an assay.