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Paper-based microreactor array for rapid screening of cell signaling cascades
- Source :
- Lab on a Chip. 16:2911-2920
- Publication Year :
- 2016
- Publisher :
- Royal Society of Chemistry (RSC), 2016.
-
Abstract
- Investigation of cell signaling pathways is important for the study of pathogenesis of cancer. However, the related operations used in these studies are time consuming and labor intensive. Thus, the development of effective therapeutic strategies may be hampered. In this work, gel-free cell culture and subsequent immunoassay has been successfully integrated and conducted in a paper-based microreactor array. Study of the activation level of different kinases of cells stimulated by different conditions, i.e., IL-6 stimulation, starvation, and hypoxia, was demonstrated. Moreover, rapid screening of cell signaling cascades after the stimulations of HGF, doxorubicin, and UVB irradiation was respectively conducted to simultaneously screen 40 kinases and transcription factors. Activation of multi-signaling pathways could be identified and the correlation between signaling pathways was discussed to provide further information to investigate the entire signaling network. The present technique integrates most of the tedious operations using a single paper substrate, reduces sample and reagent consumption, and shortens the time required by the entire process. Therefore, it provides a first-tier rapid screening tool for the study of complicated signaling cascades. It is expected that the technique can be developed for routine protocol in conventional biological research laboratories.
- Subjects :
- Paper
0301 basic medicine
Cell signaling
Time Factors
Biomedical Engineering
Bioengineering
Biology
01 natural sciences
Biochemistry
03 medical and health sciences
Bioreactors
Cell Line, Tumor
Neoplasms
Humans
Transcription factor
Cell Proliferation
Immunoassay
Kinase
Liver Neoplasms
010401 analytical chemistry
Hydrogels
Equipment Design
General Chemistry
Paper based
Microarray Analysis
0104 chemical sciences
Cell biology
030104 developmental biology
Cell culture
Immunology
Computer-Aided Design
Microreactor
Signal transduction
Cell signaling pathways
Signal Transduction
Subjects
Details
- ISSN :
- 14730189 and 14730197
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Lab on a Chip
- Accession number :
- edsair.doi.dedup.....a42651b5e8b58f55e116c349ef69ecda