Back to Search
Start Over
Evaluation of the Illumina ForenSeq™ DNA Signature Prep Kit - MPS forensic application for the MiSeq FGx™ benchtop sequencer
- Source :
- Forensic science international. Genetics. 28
- Publication Year :
- 2016
-
Abstract
- Massively Parallel (Next Generation) Sequencing (MPS) technologies have recently been proven useful and successful in typing various markers relevant in forensic genetics, such as STRs, SNPs and mitochondrial genomes. Early studies investigated self-developed DNA libraries, commercially supplied kits are currently being made available to allow a smoother and gradual implementation of such technologies in forensic laboratories. The ForenSeq™ DNA Signature Prep Kit (Illumina, CA) is the first commercially available STR kit that can be used on the MiSeq FGx™ (Illumina, CA) benchtop high-throughput sequencer. This kit allows the simultaneous typing of 59 STRs and up to 172 SNPs in a single reaction and presents a short library preparation protocol adapted to contemporary forensic requirements. In this study, we evaluated the beta version of the ForenSeq DNA Signature Prep Kit MiSeq FGx system by investigating reproducibility, sensitivity, mixtures, concordance, casework-type and aDNA samples and found it to perform successfully, proving to be a robust method for future forensic applications. MPS brings the possibility of complex multiplexing, high sensitivity and sequencing resolution to forensics; however, the need for consensual directions on databasing, data storage and nomenclature must be taken into consideration.
- Subjects :
- 0301 basic medicine
Genetics
Forensic Genetics
Male
Chromosomes, Human, X
Chromosomes, Human, Y
Library preparation
High-Throughput Nucleotide Sequencing
Reproducibility of Results
Computational biology
Biology
DNA Fingerprinting
Polymorphism, Single Nucleotide
Pathology and Forensic Medicine
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Microsatellite
Humans
Female
030216 legal & forensic medicine
Forensic genetics
Microsatellite Repeats
Subjects
Details
- ISSN :
- 18780326
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Forensic science international. Genetics
- Accession number :
- edsair.doi.dedup.....a3e3ffdadd81501d6d7ed764e49ada0a