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First Gut Content Analysis of 4th Instar Midge Larvae (Diptera: Chronomidae) In Large-Scale Weirs Using a DNA Meta-Barcoding Approach
- Source :
- International Journal of Environmental Research and Public Health, Vol 17, Iss 2856, p 2856 (2020), International Journal of Environmental Research and Public Health, Volume 17, Issue 8
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Chironomidae larvae play an important role in the food chain of river ecosystems in Korea, where it is dominant. However, detailed information on the diet of Chironomidae larvae are still lacking. The purpose of this study was to identify the gut contents of 4th instar larvae of a Chironomidae inhabiting four large-scale weirs (Sejong Weir, Juksan Weir, Gangjeong-Goryeong Weir, and Dalseong Weir) using a DNA meta-barcoding approach. We found that dominant Operational Taxonomic Unit (OUT) was assigned to Paractinolaimus sp. (Nematoda), and the sub-dominant OTU was assigned to Dicrotendipes fumidus (Chironomidae). The most common OTUs among the individuals included phytoplankton, such as Tetrahymena sp., D. armatus, Pseudopediastrum sp., Tetradesmus dimorphus, Biddulphia tridens, and Desmodesmus spp. We calculated the selectivity index (E&rsquo<br />) and provided scientific evidence that Chironomidae larvae have a significant preference (E&rsquo<br />&gt<br />0.5) for Desmodesmus armatus, E. minima, and T. dimorphus, while it does not show preference for other species found in its gut. Differences in physico-chemical factors, such as water quality, nutrients, Chl-a, and carbon concentrations, resulting from anthropogenic impacts (i.e., construction of large-scale weirs) as well as the particle size of prey organisms (small-sized single cell) and effects of chemicals (chemokinesis) could affect the feeding behavior of Chironomidae larvae.
- Subjects :
- 0106 biological sciences
Operational taxonomic unit
Health, Toxicology and Mutagenesis
Zoology
feeding behavior
lcsh:Medicine
Chironomidae larvae
010603 evolutionary biology
01 natural sciences
Chironomidae
Article
Republic of Korea
Animals
DNA Barcoding, Taxonomic
gut content
Dicrotendipes
Ecosystem
Larva
biology
010604 marine biology & hydrobiology
lcsh:R
Public Health, Environmental and Occupational Health
Desmodesmus
biology.organism_classification
DNA meta-barcoding
Gastrointestinal Microbiome
large-scale weirs
Midge
Weir
Instar
Subjects
Details
- Language :
- English
- ISSN :
- 16617827 and 16604601
- Volume :
- 17
- Issue :
- 2856
- Database :
- OpenAIRE
- Journal :
- International Journal of Environmental Research and Public Health
- Accession number :
- edsair.doi.dedup.....a3e3ce8bc48de2e9c74055b8e4c1a37b