Back to Search
Start Over
Practical Method for the Multigram Separation of the 5- and 6-Isomers of Carboxyfluorescein
- Source :
- Bioconjugate Chemistry. 8:495-497
- Publication Year :
- 1997
- Publisher :
- American Chemical Society (ACS), 1997.
-
Abstract
- An efficient preparative method for separating 5- and 6-carboxyfluorescein is presented. 6-Carboxyfluorescein dipivalate is isolated as its diisopropylamine salt, which can be converted to the free acid or used directly in coupling reactions. The 5-isomer is isolated from the acidified mother liquor. Isomerically pure carboxyfluoresceins are prepared by hydrolysis of the corresponding dipivalates.
- Subjects :
- Magnetic Resonance Spectroscopy
Free acid
Biomedical Engineering
Pharmaceutical Science
Salt (chemistry)
Bioengineering
Mass Spectrometry
Coupling reaction
chemistry.chemical_compound
Hydrolysis
fluids and secretions
Isomerism
polycyclic compounds
Organic chemistry
heterocyclic compounds
Mother liquor
Chromatography, High Pressure Liquid
Pharmacology
chemistry.chemical_classification
Chromatography
Organic Chemistry
technology, industry, and agriculture
food and beverages
Diisopropylamine
Nuclear magnetic resonance spectroscopy
Fluoresceins
chemistry
Biotechnology
Subjects
Details
- ISSN :
- 15204812 and 10431802
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Bioconjugate Chemistry
- Accession number :
- edsair.doi.dedup.....a3c88cdf7c1266c8b988eccc9f5cf06c