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Biological activities of marine invertebrates extracts from the northeast brazilian coast
Biological activities of marine invertebrates extracts from the northeast brazilian coast
- Source :
- Brazilian Journal of Biology, Issue: ahead, Published: 05 AUG 2019, Brazilian Journal of Biology v.80 n.2 2020, Brazilian Journal of Biology, Instituto Internacional de Ecologia (IIE), instacron:IIE
- Publication Year :
- 2019
- Publisher :
- Instituto Internacional de Ecologia, 2019.
-
Abstract
- This paper reports the in vitro antiproliferative effects, antiprotozoal, anti-herpes and antimicrobial activities of 32 organic extracts of 14 marine sponges and 14 corals collected in northeast Brazilian coast. The ethanolic extracts of the sponges Amphimedon compressa and Tedania ignis, and the acetone extract of Dysidea sp. showed relevant results concerning the antiproliferative effects against A549, HCT-8, and PC-3 cell lines by sulforhodamine B assay, but also low specificity. Concerning the antiprotozoal screening, the ethanolic extract of Amphimedon compressa and the acetone and ethanolic extracts of Dysidea sp. were the most active against Leishmania amazonensis and Trypanosoma cruzi expressing β-galactosidase in THP-1 cells. In the preliminary anti-HSV-1 (KOS strain) screening, the ethanolic extracts of the sponges Amphimedon compressa, Haliclona sp. and Chondrosia collectrix inhibited viral replication by more than 50%. The most promising anti-herpes results were observed for the ethanolic extract of Haliclona sp. showing high selective indices against HSV-1, KOS and 29R strains (SI> 50 and >79, respectively), and HSV-2, 333 strain (IS>108). The results of the antibacterial screening indicated that only the ethanolic extract of Amphimedon compressa exhibited a weak activity against Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli by the disk diffusion method. In view of these results, the extracts of Amphimedon compressa, Tedania ignis and Dysidea sp. were selected for further studies aiming the isolation and identification of the bioactive compounds with antiproliferative and/or antiprotozoal activities. The relevant anti-herpes activity of the ethanolic extract of Haliclona sp. also deserves special attention, and will be further investigated. Resumo Este artigo reporta as atividades in vitro antiproliferativa, atiprotozoárica, anti-herpética e antimicrobiana de 32 extratos orgânicos provenientes de 14 esponjas marinhas e 14 corais coletados no litoral nordestino brasileiro. Os extratos etanólicos das esponjas Amphimedon compressa e Tedania ignis, e o extrato acetônico de Dysidea sp. demonstraram resultados promissores em relação aos efeitos antiproliferativos frente as linhagens celulares A549, HCT-8, PC-3 pelo método da sulforrodamina B, mas sem especificidade. Em relação à atividade antiprotozárica, os extratos etanólico de Amphimedon compressa e acetônico e etanólico de Dysidea sp. apresentaram atividade contra Leishmania amazonensis e Trypanosoma cruzi através do método de expressão de β-galactosidase em células THP-1. Na investigação preliminar de atividade antiviral frente ao vírus Herpes simplex tipo 1 (cepa KOS), os extratos etanólicos das esponjas Amphimedon compressa, Haliclona sp. e Chondrosia collectrix inibiram mais de 50% da replicação viral. O extrato etanólico da esponja Haliclona sp. demonstrou resultados promissores para atividade anti-herpética com altos índices de seletividade para as cepas KOS (IS >50) e 29R (IS>79) frente ao VHS-1 e cepa 333 (IS>108) frente ao VHS-2. O extrato etanólico da esponja Amphimedon compressa exibiu uma pequena atividade contra Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli pelo método de difusão em disco. De acordo com os resultados apresentados, os extratos das esponjas Amphimedon compressa, Tedania ignis e Dysidea sp. serão selecionados para futuros estudos de isolamento e identificação dos compostos bioativos para as atividades antiproliferativa e antiprozoárica. O extrato etanólico de Haliclona sp. será investigado por possuir atividade relevante anti-herpética.
- Subjects :
- atividade antimicrobiana
marine invertebrates
QH301-705.5
medicine.drug_class
Science
medicine.disease_cause
01 natural sciences
Enterococcus faecalis
atividade antiprotozoárica
anti-herpes activity
atividade antiproliferativa
medicine
Animals
Agar diffusion test
Amphimedon compressa
Biology (General)
Trypanosoma cruzi
Escherichia coli
antimicrobial activity
biology
Traditional medicine
Plant Extracts
010405 organic chemistry
antiproliferative effects
antiprotozoal activity
Botany
Marine invertebrates
biology.organism_classification
Antimicrobial
Anti-Bacterial Agents
Porifera
0104 chemical sciences
atividade anti-herpética
010404 medicinal & biomolecular chemistry
QL1-991
invertebrados marinhos
QK1-989
Antiprotozoal
General Agricultural and Biological Sciences
Zoology
Brazil
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Brazilian Journal of Biology, Issue: ahead, Published: 05 AUG 2019, Brazilian Journal of Biology v.80 n.2 2020, Brazilian Journal of Biology, Instituto Internacional de Ecologia (IIE), instacron:IIE
- Accession number :
- edsair.doi.dedup.....a3b644a10c36ecd5c3b516fff70af5df