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Robust correlations across six SARS‐CoV‐2 serology assays detecting distinct antibody features
- Source :
- Clinical & Translational Immunology, Clinical & Translational Immunology, Vol 10, Iss 3, Pp n/a-n/a (2021), Clinical & translational immunology, 10(3). Nature Publishing Group, Clinical & Translational Immunology, 10(3):e1258
- Publication Year :
- 2021
-
Abstract
- Objectives As the world transitions into a new era of the COVID‐19 pandemic in which vaccines become available, there is an increasing demand for rapid reliable serological testing to identify individuals with levels of immunity considered protective by infection or vaccination. Methods We used 34 SARS‐CoV‐2 samples to perform a rapid surrogate virus neutralisation test (sVNT), applicable to many laboratories as it circumvents the need for biosafety level‐3 containment. We correlated results from the sVNT with five additional commonly used SARS‐CoV‐2 serology techniques: the microneutralisation test (MNT), in‐house ELISAs, commercial Euroimmun‐ and Wantai‐based ELISAs (RBD, spike and nucleoprotein; IgG, IgA and IgM), antigen‐binding avidity, and high‐throughput multiplex analyses to profile isotype, subclass and Fc effector binding potential. We correlated antibody levels with antibody‐secreting cell (ASC) and circulatory T follicular helper (cTfh) cell numbers. Results Antibody data obtained with commercial ELISAs closely reflected results using in‐house ELISAs against RBD and spike. A correlation matrix across ten measured ELISA parameters revealed positive correlations for all factors. The frequency of inhibition by rapid sVNT strongly correlated with spike‐specific IgG and IgA titres detected by both commercial and in‐house ELISAs, and MNT titres. Multiplex analyses revealed strongest correlations between IgG, IgG1, FcR and C1q specific to spike and RBD. Acute cTfh‐type 1 cell numbers correlated with spike and RBD‐specific IgG antibodies measured by ELISAs and sVNT. Conclusion Our comprehensive analyses provide important insights into SARS‐CoV‐2 humoral immunity across distinct serology assays and their applicability for specific research and/or diagnostic questions to assess SARS‐CoV‐2‐specific humoral responses.<br />We describe robust correlations across six SARS‐CoV‐2 serology assays detecting distinct antibody features. Our comprehensive analyses provide important insights into SARS‐CoV‐2 humoral immunity across distinct serology assays and their applicability for specific research and/or diagnostic questions to assess SARS‐CoV‐2‐specific humoral responses.
- Subjects :
- 0301 basic medicine
lcsh:Immunologic diseases. Allergy
Immunology
Subclass
Serology
03 medical and health sciences
0302 clinical medicine
Immunology and Allergy
Multiplex
Avidity
COVID-19
neutralisation assay
SARS-CoV-2 antibodies
ELISA
T follicular helper cells
antibody-secreting cells
030212 general & internal medicine
General Nursing
antibody‐secreting cells
biology
SARS‐CoV‐2 antibodies
Isotype
3. Good health
Vaccination
030104 developmental biology
Humoral immunity
biology.protein
Original Article
Antibody
lcsh:RC581-607
Subjects
Details
- ISSN :
- 20500068
- Database :
- OpenAIRE
- Journal :
- Clinical & Translational Immunology
- Accession number :
- edsair.doi.dedup.....a3996f5eee8339ed6ecc197af4c75096
- Full Text :
- https://doi.org/10.1002/cti2.1258