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Extracellular hydrocarbon and intracellular lipid accumulation are related to nutrient-sufficient conditions in pH-controlled chemostat cultures of the microalga Botryococcus braunii SAG 30.81

Authors :
Jian Jin
Dominique Grizeau
Catherine Dupré
Jack Legrand
Laboratoire de génie des procédés - environnement - agroalimentaire (GEPEA)
Mines Nantes (Mines Nantes)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST)
Université de Nantes (UN)-Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS)
Source :
Algal Research-Biomass, Biofuels and Bioproducts, Algal Research-Biomass, Biofuels and Bioproducts, Elsevier, 2016, 17, pp.244-252. ⟨10.1016/j.algal.2016.05.007⟩
Publication Year :
2016
Publisher :
HAL CCSD, 2016.

Abstract

International audience; Here we investigate the effect of environmental pH on biomass and hydrocarbon productivity in photoautotro-phic cultures of Botryococcus braunii SAG 30.81 (race A). Successive steady-states of continuous cultures were used to study the effects of pH controlled by carbon dioxide feeds in a closed photobioreactor. At a fixed dilution rate of 0.2 d −1 , hydrocarbon productivity was found to be pH-dependent in the range 5.5 to 8.0, with an optimum at pH 6.5. pH variations from pH 6.0 to pH 8.0 had little effect on biomass productivity, nutrient requirement for biomass production, photosynthetic yield and pigment ratios, whereas acidic pH 5.5 conditions induced a slight decrease in biomass and hydrocarbon productivities as well as stressing the cells. The pH effect occurred even after a threefold increase in nitrate and phosphate concentrations in the feed culture medium, resulting in bio-mass productivity up to 8.9 g m −2 d −1 and hydrocarbon productivity up to 0.5 g m −2 d −1. Dissolved inorganic carbon data suggest that a maximum hydrocarbon content of 5.7% dry weight was reached at and above 1 mmol L −1 dissolved CO 2 and that hydrocarbon production was linearly related to CO 2 up to 2.5 mmol L −1 , whatever the N and P enrichment of AF-6 culture medium.

Details

Language :
English
ISSN :
22119264
Database :
OpenAIRE
Journal :
Algal Research-Biomass, Biofuels and Bioproducts, Algal Research-Biomass, Biofuels and Bioproducts, Elsevier, 2016, 17, pp.244-252. ⟨10.1016/j.algal.2016.05.007⟩
Accession number :
edsair.doi.dedup.....a37639d4869fd41f7f185a0601783fbb
Full Text :
https://doi.org/10.1016/j.algal.2016.05.007⟩