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Extracellular hydrocarbon and intracellular lipid accumulation are related to nutrient-sufficient conditions in pH-controlled chemostat cultures of the microalga Botryococcus braunii SAG 30.81
- Source :
- Algal Research-Biomass, Biofuels and Bioproducts, Algal Research-Biomass, Biofuels and Bioproducts, Elsevier, 2016, 17, pp.244-252. ⟨10.1016/j.algal.2016.05.007⟩
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- International audience; Here we investigate the effect of environmental pH on biomass and hydrocarbon productivity in photoautotro-phic cultures of Botryococcus braunii SAG 30.81 (race A). Successive steady-states of continuous cultures were used to study the effects of pH controlled by carbon dioxide feeds in a closed photobioreactor. At a fixed dilution rate of 0.2 d −1 , hydrocarbon productivity was found to be pH-dependent in the range 5.5 to 8.0, with an optimum at pH 6.5. pH variations from pH 6.0 to pH 8.0 had little effect on biomass productivity, nutrient requirement for biomass production, photosynthetic yield and pigment ratios, whereas acidic pH 5.5 conditions induced a slight decrease in biomass and hydrocarbon productivities as well as stressing the cells. The pH effect occurred even after a threefold increase in nitrate and phosphate concentrations in the feed culture medium, resulting in bio-mass productivity up to 8.9 g m −2 d −1 and hydrocarbon productivity up to 0.5 g m −2 d −1. Dissolved inorganic carbon data suggest that a maximum hydrocarbon content of 5.7% dry weight was reached at and above 1 mmol L −1 dissolved CO 2 and that hydrocarbon production was linearly related to CO 2 up to 2.5 mmol L −1 , whatever the N and P enrichment of AF-6 culture medium.
- Subjects :
- 0106 biological sciences
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Hydrocarbon
020209 energy
Botryococcus
Biomass
Photobioreactor
02 engineering and technology
Chemostat
7. Clean energy
01 natural sciences
chemistry.chemical_compound
Continuous culture
010608 biotechnology
Botany
pH effect
0202 electrical engineering, electronic engineering, information engineering
Botryococcus braunii
[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
biology
Chemistry
biology.organism_classification
Phosphate
Lipids
Dilution
Environmental chemistry
Dissolved CO 2
Carbon dioxide
Agronomy and Crop Science
Subjects
Details
- Language :
- English
- ISSN :
- 22119264
- Database :
- OpenAIRE
- Journal :
- Algal Research-Biomass, Biofuels and Bioproducts, Algal Research-Biomass, Biofuels and Bioproducts, Elsevier, 2016, 17, pp.244-252. ⟨10.1016/j.algal.2016.05.007⟩
- Accession number :
- edsair.doi.dedup.....a37639d4869fd41f7f185a0601783fbb
- Full Text :
- https://doi.org/10.1016/j.algal.2016.05.007⟩