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Sensitization and Elicitation of an Allergic Reaction to Wheat Gliadins in Mice
- Source :
- Journal of Agricultural and Food Chemistry, Journal of Agricultural and Food Chemistry, American Chemical Society, 2009, 57 (4), pp.1219-1225. ⟨10.1021/jf802898u⟩
- Publication Year :
- 2009
- Publisher :
- American Chemical Society (ACS), 2009.
-
Abstract
- International audience; We developed a mouse model of allergy to wheat flour gliadins, a protein fraction containing major wheat allergens. We compared the antibody responses (i.e., specific IgE and IgG1) and the profiles of cytokines secreted by reactivated splenocytes induced after intraperitoneal injections of gliadins in three strains of mice, namely, Balb/cJ, B10.A, and C3H/HeJ. The intensities of the allergic reactions elicited by intranasal challenge were also compared. Both the sensitization and elicitation were the highest in Balb/cJ mice, whereas weak or no reaction was observed in the others strains. Interestingly, the specificity of the mouse IgE against the different gliadins (i.e., alpha-, beta-, gamma-, omega 1,2-, and omega 5-gliadin) was similar to that observed in children allergic to wheat flour. Balb/cJ mice may thus provide a relevant model for the study of sensitization and elicitation by wheat gliadins and for improving our understanding of the specific role and mechanisms of action of the different classes of gliadins.
- Subjects :
- Allergy
Immunoglobulin E
Gliadin
Mice
0302 clinical medicine
Antibody Specificity
[SDV.IDA]Life Sciences [q-bio]/Food engineering
Child
Triticum
Sensitization
Mice, Inbred BALB C
Mice, Inbred C3H
0303 health sciences
biology
Chemistry
INDUCTION
food and beverages
MOUSE MODEL
3. Good health
ALLERGY
medicine.anatomical_structure
Plant protein
Cytokines
Female
IgE
Antibody
General Agricultural and Biological Sciences
Adult
IgG
WHEAT
Wheat flour
03 medical and health sciences
Th2 Cells
GLIADINS
Hypersensitivity
Splenocyte
medicine
Animals
Humans
030304 developmental biology
nutritional and metabolic diseases
General Chemistry
medicine.disease
digestive system diseases
Eosinophils
Disease Models, Animal
030228 respiratory system
Immunoglobulin G
Immunology
biology.protein
Immunization
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 57
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi.dedup.....a36d2238f0e77defee60be5486d067a3