Back to Search
Start Over
Identification of the Red-Necked Longhorn Beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with real-Time PCR on frass
- Source :
- Sustainability (Basel) 12 (2020). doi:10.3390/su12156041, info:cnr-pdr/source/autori:Rizzo D., Taddei A., Da Lio D., Nugnes F., Barra E., Stefani L., Bartolini L., Griffo R.V., Spigno P., Cozzolino L., Rossi E., Garonna A.P./titolo:Identification of the Red-Necked Longhorn Beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with real-Time PCR on frass/doi:10.3390%2Fsu12156041/rivista:Sustainability (Basel)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:12, Sustainability, Volume 12, Issue 15, Sustainability, Vol 12, Iss 6041, p 6041 (2020)
- Publication Year :
- 2020
- Publisher :
- MDPI, Basel, 2020.
-
Abstract
- Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae), the red-necked longhorn beetle is native to eastern Asia, where it is an important wood-borer of fruit and ornamental species of the genus Prunus. A. bungii is a quarantine pest in the European Union, following its accidental introduction and establishment in Germany and Italy, and is currently included in the list of priority pests. To confirm its infestations in outbreak areas, adult or larval specimens are needed to perform morphological or molecular analyses. The presence of A. bungii larvae inside the attacked trees makes the collection of specimens particularly difficult. Thus, we present two diagnostic protocols based on frass analysis with real-time PCR (probe and SYBR Green). The results obtained show that a non-invasive approach for detecting the presence of this harmful invasive pest can be a reliable and accurate alternative diagnostic tool in phytosanitary surveys, as well as to outline a sustainable pest management strategy.
- Subjects :
- 0106 biological sciences
Integrated pest management
non-invasive diagnostic tool
Geography, Planning and Development
TJ807-830
Zoology
Management, Monitoring, Policy and Law
Biology
TD194-195
01 natural sciences
Renewable energy sources
law.invention
03 medical and health sciences
Prunus
law
Quarantine
media_common.cataloged_instance
GE1-350
European union
030304 developmental biology
Phytosanitary certification
media_common
xylophagous insect
0303 health sciences
Environmental effects of industries and plants
Renewable Energy, Sustainability and the Environment
quarantine pest
Frass
Frass DNA, Non-invasive diagnostic tool, Phytosanitary survey, Quarantine pest, Xylophagous insect
fungi
food and beverages
Environmental sciences
010602 entomology
phytosanitary survey
frass DNA
PEST analysis
Longhorn beetle
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Sustainability (Basel) 12 (2020). doi:10.3390/su12156041, info:cnr-pdr/source/autori:Rizzo D., Taddei A., Da Lio D., Nugnes F., Barra E., Stefani L., Bartolini L., Griffo R.V., Spigno P., Cozzolino L., Rossi E., Garonna A.P./titolo:Identification of the Red-Necked Longhorn Beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with real-Time PCR on frass/doi:10.3390%2Fsu12156041/rivista:Sustainability (Basel)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:12, Sustainability, Volume 12, Issue 15, Sustainability, Vol 12, Iss 6041, p 6041 (2020)
- Accession number :
- edsair.doi.dedup.....a368d4faa2fa08dd8041f94b1c952a85
- Full Text :
- https://doi.org/10.3390/su12156041