Back to Search Start Over

Identification of the Red-Necked Longhorn Beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with real-Time PCR on frass

Authors :
Eleonora Barra
L. Stefani
Francesco Nugnes
Paola Spigno
Antonio P. Garonna
Linda Bartolini
Domenico Rizzo
Raffaele Griffo
Elisabetta Rossi
Andrea Taddei
Daniele Da Lio
Lucia Cozzolino
Rizzo, D.
Taddei, A.
Da Lio, D.
Nugnes, F.
Barra, E.
Stefani, L.
Bartolini, L.
Griffo, R. V.
Spigno, P.
Cozzolino, L.
Rossi, E.
Garonna, A. P.
Source :
Sustainability (Basel) 12 (2020). doi:10.3390/su12156041, info:cnr-pdr/source/autori:Rizzo D., Taddei A., Da Lio D., Nugnes F., Barra E., Stefani L., Bartolini L., Griffo R.V., Spigno P., Cozzolino L., Rossi E., Garonna A.P./titolo:Identification of the Red-Necked Longhorn Beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with real-Time PCR on frass/doi:10.3390%2Fsu12156041/rivista:Sustainability (Basel)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:12, Sustainability, Volume 12, Issue 15, Sustainability, Vol 12, Iss 6041, p 6041 (2020)
Publication Year :
2020
Publisher :
MDPI, Basel, 2020.

Abstract

Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae), the red-necked longhorn beetle is native to eastern Asia, where it is an important wood-borer of fruit and ornamental species of the genus Prunus. A. bungii is a quarantine pest in the European Union, following its accidental introduction and establishment in Germany and Italy, and is currently included in the list of priority pests. To confirm its infestations in outbreak areas, adult or larval specimens are needed to perform morphological or molecular analyses. The presence of A. bungii larvae inside the attacked trees makes the collection of specimens particularly difficult. Thus, we present two diagnostic protocols based on frass analysis with real-time PCR (probe and SYBR Green). The results obtained show that a non-invasive approach for detecting the presence of this harmful invasive pest can be a reliable and accurate alternative diagnostic tool in phytosanitary surveys, as well as to outline a sustainable pest management strategy.

Details

Language :
English
Database :
OpenAIRE
Journal :
Sustainability (Basel) 12 (2020). doi:10.3390/su12156041, info:cnr-pdr/source/autori:Rizzo D., Taddei A., Da Lio D., Nugnes F., Barra E., Stefani L., Bartolini L., Griffo R.V., Spigno P., Cozzolino L., Rossi E., Garonna A.P./titolo:Identification of the Red-Necked Longhorn Beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with real-Time PCR on frass/doi:10.3390%2Fsu12156041/rivista:Sustainability (Basel)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:12, Sustainability, Volume 12, Issue 15, Sustainability, Vol 12, Iss 6041, p 6041 (2020)
Accession number :
edsair.doi.dedup.....a368d4faa2fa08dd8041f94b1c952a85
Full Text :
https://doi.org/10.3390/su12156041