Back to Search
Start Over
Sandwich hybridization probes for the detection of Pseudo-nitzschia (Bacillariophyceae) species: An update to existing probes and a description of new probes
- Source :
- Harmful Algae. 70:37-51
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- New sandwich hybridization assay (SHA) probes for detecting Pseudo-nitzschia species (P. arenysensis, P. fraudulenta, P. hasleana, P. pungens) are presented, along with updated cross-reactivity information on historical probes (SHA and FISH; fluorescence in situ hybridization) targeting P. australis and P. multiseries. Pseudo-nitzschia species are a cosmopolitan group of diatoms that produce varying levels of domoic acid (DA), a neurotoxin that can accumulate in finfish and shellfish and transfer throughout the food web. Consumption of infected food sources can lead to illness in humans (amnesic shellfish poisoning; ASP) and marine wildlife (domoic acid poisoning; DAP). The threat of human illness, along with economic loss from fishery closures has resulted in the implementation of monitoring protocols and intensive ecological studies. SHA probes have been instrumental in some of these efforts, as the technique performs well in complex heterogeneous sample matrices and has been adapted to benchtop and deployable (Environmental Sample Processor) platforms. The expanded probe set will enhance future efforts towards understanding spatial, temporal and successional patterns in species during bloom and non-bloom periods.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Neurotoxins
Zoology
Plant Science
Aquatic Science
Sensitivity and Specificity
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Amnesic shellfish poisoning
medicine
Shellfish
Diatoms
Heterogeneous sample
Kainic Acid
medicine.diagnostic_test
biology
Ecology
010604 marine biology & hydrobiology
Nucleic Acid Hybridization
Domoic acid
biology.organism_classification
Food web
030104 developmental biology
chemistry
Molecular Probes
Molecular probe
Pseudo-nitzschia
Fluorescence in situ hybridization
Subjects
Details
- ISSN :
- 15689883
- Volume :
- 70
- Database :
- OpenAIRE
- Journal :
- Harmful Algae
- Accession number :
- edsair.doi.dedup.....a36260cc7095dc89bf1dc0bb6c508c5b