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Expression of β4 Integrins in Human Skin: Comparison of Epidermal Distribution with β1-Integrin Epitopes, and Modulation by Calcium and Vitamin D3 in Cultured Keratinocytes
- Source :
- Journal of Investigative Dermatology. 97:562-567
- Publication Year :
- 1991
- Publisher :
- Elsevier BV, 1991.
-
Abstract
- The expression of beta 4 integrins in adult and fetal human skin as well as in cultured keratinocytes was studied by immunodetection with monoclonal antibodies, and compared with that of beta 1 integrins. The distribution of the beta 1 and beta 4 integrin epitopes was entirely different in the adult epidermis. As reported previously by us (J Clin Invest 84:1916, 1989), the beta 1 epitopes were present most prominently at lateral cell-cell contact points, whereas staining with the beta 4 antibody demonstrated a linear staining pattern polarizing to the basal surface juxtaposed to the dermal-epidermal basement membrane. In contrast, in fetal skin, the staining patterns both with beta 1 and beta 4 antibodies were similar and demonstrated the presence of epitopes surrounding the entire cell surface of both basal and suprabasal keratinocytes. Distinct polarization of beta 4 integrin epitopes was noted in cultured keratinocytes maintained in low-calcium (0.15 mM) medium, and the expression of these integrins was also noted in human papilloma virus-transformed keratinocytes. Switch of the cultures to high-calcium (1.2 mM) medium resulted in redistribution of the epitopes to a pattern suggesting their location at under-surface of the cells adjacent to the substratum. This Ca(++)-induced redistribution of beta 4 integrin epitopes could be counteracted by 10(-7) M vitamin D3. Finally, incubation with anti-beta 4 integrin antibody reduced the capacity of keratinocytes to attach to plastic substratum. The results provide further evidence for the role of beta 4 integrins in cell-matrix interactions.
- Subjects :
- Adult
Keratinocytes
Integrins
medicine.drug_class
Integrin
Human skin
Tris-buffered saline
Dermatology
Biology
Monoclonal antibody
Biochemistry
Epitope
Epitopes
chemistry.chemical_compound
medicine
Humans
Molecular Biology
Cells, Cultured
Cholecalciferol
Skin
Basement membrane
Infant
Cell Biology
Molecular biology
medicine.anatomical_structure
chemistry
Cell culture
biology.protein
Calcium
Keratinocyte
Subjects
Details
- ISSN :
- 0022202X
- Volume :
- 97
- Database :
- OpenAIRE
- Journal :
- Journal of Investigative Dermatology
- Accession number :
- edsair.doi.dedup.....a34c97616c5e09a2de1ccc682d490614