Back to Search
Start Over
The Dynamic Transcriptional Cell Atlas of Testis Development during Human Puberty
- Source :
- Guo, J, Nie, X, Giebler, M, Mlcochova, H, Wang, Y, Grow, E J, DonorConnect, Kim, R, Tharmalingam, M, Matilionyte, G, Lindskog, C, Carrell, D T, Mitchell, R T, Goriely, A, Hotaling, J M & Cairns, B R 2020, ' The Dynamic Transcriptional Cell Atlas of Testis Development during Human Puberty ', Cell Stem Cell, vol. 26, no. 2, pp. 262-276.e4 . https://doi.org/10.1016/j.stem.2019.12.005, Cell Stem Cell
- Publication Year :
- 2020
-
Abstract
- Summary The human testis undergoes dramatic developmental and structural changes during puberty, including proliferation and maturation of somatic niche cells, and the onset of spermatogenesis. To characterize this understudied process, we profiled and analyzed single-cell transcriptomes of ∼10,000 testicular cells from four boys spanning puberty and compared them to those of infants and adults. During puberty, undifferentiated spermatogonia sequentially expand and differentiate prior to the initiation of gametogenesis. Notably, we identify a common pre-pubertal progenitor for Leydig and myoid cells and delineate candidate factors controlling pubertal differentiation. Furthermore, pre-pubertal Sertoli cells exhibit two distinct transcriptional states differing in metabolic profiles before converging to an alternative single mature population during puberty. Roles for testosterone in Sertoli cell maturation, antimicrobial peptide secretion, and spermatogonial differentiation are further highlighted through single-cell analysis of testosterone-suppressed transfemale testes. Taken together, our transcriptional atlas of the developing human testis provides multiple insights into developmental changes and key factors accompanying male puberty.<br />Graphical Abstract<br />Highlights • A transcriptional single-cell atlas of the developing testes during human puberty • Distinctive phases of germ cell differentiation occur during puberty • Identification of a common progenitor for Leydig and myoid cells prior to puberty • Partial reversal of Sertoli and germ cell maturation upon testosterone suppression<br />Guo et al. provide a transcriptional cell atlas of the developing human testis during puberty, revealing dramatic developmental changes in both germ and somatic niche cell lineages. Furthermore, germ cells and Sertoli cells from testosterone-suppressed transfemale testes display partial developmental reversal, revealing critical roles for testosterone in maintaining testis maturation.
- Subjects :
- Adult
Male
puberty
Somatic cell
Cellbiologi
Cell
state 0
Biology
Article
single cell sequencing
spermatogonial stem cell
Human puberty
03 medical and health sciences
0302 clinical medicine
Leydig cell
Atlas (anatomy)
Testis
Genetics
medicine
Humans
skin and connective tissue diseases
Spermatogenesis
Gene
030304 developmental biology
0303 health sciences
Sertoli Cells
testis maturation
Infant
Cell Biology
Sertoli cell
Spermatogonia
Cell biology
medicine.anatomical_structure
Single cell sequencing
transfemale
testosterone
Molecular Medicine
sense organs
peritubular myoid cell
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Guo, J, Nie, X, Giebler, M, Mlcochova, H, Wang, Y, Grow, E J, DonorConnect, Kim, R, Tharmalingam, M, Matilionyte, G, Lindskog, C, Carrell, D T, Mitchell, R T, Goriely, A, Hotaling, J M & Cairns, B R 2020, ' The Dynamic Transcriptional Cell Atlas of Testis Development during Human Puberty ', Cell Stem Cell, vol. 26, no. 2, pp. 262-276.e4 . https://doi.org/10.1016/j.stem.2019.12.005, Cell Stem Cell
- Accession number :
- edsair.doi.dedup.....a34c92b065772a270fb984c1b4f55033