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Different expression of lipocalin-type prostaglandin D synthase in rat epididymidis
- Source :
- Animal Reproduction Science. 98:302-310
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- This study was designed to explore the different expression of L-PGDS (lipocalin-type prostaglandin D synthase) in rat epididymidis and to gain further insight into the potential function of L-PGDS in male reproduction. The expression of L-PGDS in rat epididymidis was assessed using real-time quantitative PCR and immunoblotting. The distribution of L-PGDS in rat epididymidis was explored by immunohistochemical methods. The result of immunohistochemistry displayed that L-PGDS was mainly distributed in epididymidis and localized within the cytoplasm and the cilia of the epithelial cells. Real-time quantitative PCR and immunoblotting showed that L-PGDS was strikingly expressed in the caput epididymidis, while a moderate to weak expression was observed in the corpus and cauda epididymidis, the level of mRNA was 0.52 ± 0.02 in the caput, 0.48 ± 0.03 in the corpus and 0.32 ± 0.01 in the cauda epididymidis, the level of protein expression in caput, corpus and the cauda groups was 1, 0.89 ± 0.03 and 0.62 ± 0.01, which suggested that L-PGDS may play certain kind of role during the process of the spermatozoa maturation.
- Subjects :
- Male
endocrine system
medicine.medical_specialty
Blotting, Western
Gene Expression
Prostaglandin-D synthase
Caput epididymidis
Rats, Sprague-Dawley
Andrology
Endocrinology
Food Animals
Internal medicine
medicine
Animals
Lipocalin-type prostaglandin D synthase
RNA, Messenger
Epididymis
Messenger RNA
biology
Reverse Transcriptase Polymerase Chain Reaction
urogenital system
Cilium
General Medicine
Lipocalins
Rats
Intramolecular Oxidoreductases
Real-time polymerase chain reaction
Cytoplasm
biology.protein
Immunohistochemistry
Animal Science and Zoology
Subjects
Details
- ISSN :
- 03784320
- Volume :
- 98
- Database :
- OpenAIRE
- Journal :
- Animal Reproduction Science
- Accession number :
- edsair.doi.dedup.....a34c87f503b2754d2e9dec21d5cad30e