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Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111
- Source :
- International Journal of Molecular Sciences, Vol 22, Iss 2453, p 2453 (2021), International Journal of Molecular Sciences, Volume 22, Issue 5
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Probiotics make up a large and growing segment of the commercial market of dietary supplements and are touted as offering a variety of human health benefits. Some of the purported positive impacts of probiotics include, but are not limited to, stabilization of the gut microbiota, prevention of gastrointestinal disorders and modulation of the host immune system. Current research suggests that the immunomodulatory effects of probiotics are strain-specific and vary in mode of action. Here, we examined the immunomodulatory properties of Bacillus subtilis strain DE111 in a healthy human population. In a pilot randomized, double blind, placebo-controlled four-week intervention, we examined peripheral blood mononuclear cells (PBMCs) at basal levels pre- and post-intervention, as well as in response to stimulation with bacterial lipopolysaccharide (LPS). We observed an increase in anti-inflammatory immune cell populations in response to ex vivo LPS stimulation of PBMCs in the DE111 intervention group. Overall perceived gastrointestinal health, microbiota, and circulating and fecal markers of inflammation (Il-6, sIgA) and gut barrier function (plasma zonulin) were largely unaffected by DE111 intervention, although the study may have been underpowered to detect these differences. These pilot data provide information and justification to conduct an appropriately powered clinical study to further examine the immunomodulatory potential of B. subtilis DE111 in human populations.
- Subjects :
- 0301 basic medicine
Population
short chain fatty acid
Inflammation
Bacillus subtilis
Gut flora
Peripheral blood mononuclear cell
Catalysis
law.invention
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
Probiotic
0302 clinical medicine
Immune system
immune cells
law
medicine
Physical and Theoretical Chemistry
education
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
education.field_of_study
biology
gastrointestinal health
business.industry
flow cytometry
Organic Chemistry
Zonulin
General Medicine
biology.organism_classification
Computer Science Applications
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Immunology
peripheral blood mononuclear cells (PBMC)
medicine.symptom
business
probiotic
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Volume :
- 22
- Issue :
- 2453
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....a34c72d5520c2c29079eec530bb4637c