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New developments in corneal endothelial cell replacement
- Source :
- Acta Ophthalmologica, Acta Ophthalmologica. WILEY, Acta ophthalmologica (Oxford)
- Publication Year :
- 2020
- Publisher :
- WILEY, 2020.
-
Abstract
- Corneal transplantation is currently the most effective treatment to restore corneal clarity in patients with endothelial disorders. Endothelial transplantation, either by Descemet membrane endothelial keratoplasty (DMEK) or by Descemet stripping (automated) endothelial keratoplasty (DS(A)EK), is a surgical approach that replaces diseased Descemet membrane and endothelium with tissue from a healthy donor eye. Its application, however, is limited by the availability of healthy donor tissue. To increase the pool of endothelial grafts, research has focused on developing new treatment options as alternatives to conventional corneal transplantation. These treatment options can be considered as either 'surgery-based', that is tissue-efficient modifications of the current techniques (e.g. Descemet stripping only (DSO)/Descemetorhexis without endothelial keratoplasty (DWEK) and Quarter-DMEK), or 'cell-based' approaches, which rely on in vitro expansion of human corneal endothelial cells (hCEC) (i.e. cultured corneal endothelial cell sheet transplantation and cell injection). In this review, we will focus on the most recent developments in the field of the 'cell-based' approaches. Starting with the description of aspects involved in the isolation of hCEC from donor tissue, we then describe the different natural and bioengineered carriers currently used in endothelial cell sheet transplantation, and finally, we discuss the current 'state of the art' in novel therapeutic approaches such as endothelial cell injection.
- Subjects :
- medicine.medical_specialty
Corneal endothelium
genetic structures
Endothelium
Corneal Diseases/surgery
medicine.medical_treatment
Corneal Diseases
03 medical and health sciences
corneal endothelium
0302 clinical medicine
Tissue engineering
Cornea
Ophthalmology
cornea
Humans
Medicine
Cells, Cultured
Corneal transplantation
Endothelium, Corneal/transplantation
030304 developmental biology
0303 health sciences
business.industry
Endothelium, Corneal
General Medicine
Tissue Donors
eye diseases
Transplantation
Endothelial stem cell
medicine.anatomical_structure
tissue engineering
030221 ophthalmology & optometry
Corneal endothelial cell
Human medicine
Descemet Stripping Endothelial Keratoplasty/methods
sense organs
business
Descemet Stripping Endothelial Keratoplasty
transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 1755375X
- Database :
- OpenAIRE
- Journal :
- Acta Ophthalmologica, Acta Ophthalmologica. WILEY, Acta ophthalmologica (Oxford)
- Accession number :
- edsair.doi.dedup.....a34c2a2a50db46c0a69e8e00ac2f9c35