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Ovine CD16(+)/CD14(-) blood lymphocytes present all the major characteristics of natural killer cells
- Source :
- Veterinary Research 1 (41), 1-12. (2010), Veterinary Research, Veterinary Research, BioMed Central, 2010, 41 (1), pp.1-12. ⟨10.1051/vetres/2009052⟩
- Publication Year :
- 2010
-
Abstract
- Natural killer (NK) cells have a key role in the innate immune response against pathogens because of their cytotoxic properties and production of interferon-gamma (IFN-gamma). Some insight into ruminant NK cell biology has been gained through the characterization of bovine NK cells as NKp46(+)/CD3(-) cells. However, ovine NK cells have been little studied because of the lack of specific antibodies. Most NK cells in humans and cattle express CD16. We found that an antibody against human CD16 that cross-reacts with bovine NK cells also recognizes cell populations in ovine peripheral blood mononuclear cells. Using double labelling with CD14 revealed the same profile as described in other species, and we identified a putative NK cell population. We therefore sorted this ovine CD16(+)/CD14(-) cell population and tested it for NK cell characteristics. More than 80% of sorted CD16(+)/CD14(-) cells expressed perforin. After a week of culture in the presence of IL-2 and IL-15, ovine CD16(+)/CD14(-) cells had become large cells with intra-cytoplasmic granules containing perforin, and the vast majority displayed an activated CD2(-/low)/CD25(+)/CD8(+) phenotype, as observed for bovine NKp46(+)/CD3(-) cells. Moreover, these cells expressed transcripts for the NKp46 receptor, and were cytotoxic in a CD16-mediated redirected lysis assay against a murine cell line, P815, and in a direct lysis assay against the ovine cell line, IDO5. Finally, ovine CD16(+)/CD14(-) cells having expanded for 7 days in culture secreted IFN-gamma in response to IL-12 in a dose-dependent manner. Taken together, these findings led us to conclude that the ovine CD16(+)/CD14(-) lymphocyte sub-population displays the phenotype and functional characteristics of NK cells.
- Subjects :
- système immunitaire
Lymphocyte
Lipopolysaccharide Receptors
Interleukin 21
0302 clinical medicine
ovin
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Cytotoxic T cell
IL-2 receptor
perforin
0303 health sciences
[SDV.BA]Life Sciences [q-bio]/Animal biology
Janus kinase 3
cytotoxicité
hemic and immune systems
Interleukin-12
immunité innée
Killer Cells, Natural
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
medicine.anatomical_structure
cellule natural killer
Interleukin 12
cytotoxicity
[SDV.IMM]Life Sciences [q-bio]/Immunology
Original Article
Female
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
interferon-gamma
CD2 Antigens
chemical and pharmacologic phenomena
natural killer cell
ovine
FUNCTION-ASSOCIATED MOLECULE
NK CELLS
DENDRITIC CELLS
CYTOTOXIC LYMPHOCYTES
INNATE IMMUNITY
T-LYMPHOCYTES
MONOCYTES
ACTIVATION
EXPRESSION
SHEEP
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
Natural killer cell
03 medical and health sciences
medicine
Animals
030304 developmental biology
Sheep
Lymphokine-activated killer cell
General Veterinary
Natural Cytotoxicity Triggering Receptor 1
Receptors, IgG
Interleukin-2 Receptor alpha Subunit
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
Virology
Molecular biology
[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics
interferon gamma
Gene Expression Regulation
Cattle
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 09284249 and 12979716
- Database :
- OpenAIRE
- Journal :
- Veterinary Research 1 (41), 1-12. (2010), Veterinary Research, Veterinary Research, BioMed Central, 2010, 41 (1), pp.1-12. ⟨10.1051/vetres/2009052⟩
- Accession number :
- edsair.doi.dedup.....a34a44cb4b29b499f5a220e4da5265b1