Back to Search
Start Over
Effect of sulfation and partial hydrolysis of polysaccharides from Polygonatum sibiricum on immune-enhancement
- Source :
- International Journal of Biological Macromolecules. 122:10-18
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- The aqueous polysaccharide from Polygonatum sibiricum was extracted and fractionated using anion-exchange chromatography to obtain F1 fraction. The F1 was chemically sulfated and partially acid-hydrolyzed for the production of its over-sulfated (OS1,2,3) and hydrolyzed (HP1,2,3) derivatives, in which the sulfate content of OS1,2,3 was 7.5–17.1%, and the Mw of HP1,2,3 ranged from 18.2 × 103 to 57.3 × 103 g/mol. Considerable RAW264.7 cell activation was observed by HP1,2,3 with NO production of 34.9, 44.3 and 42.7 μM, respectively, as well as the mRNA expression of cytokines (IL-1β, IL-6, IL-10 and IL-12). NK cell cytotoxicity against HT-29 cell was facilitated by OS1,2,3 treatment with the increased gene expressions of INF-γ, Granzyme-B, perforin, NKG2D, and FasL. RAW264.7 cells appeared to be activated via MR and TLR4 mediated signaling pathway, but CR3 and TRL2 might play a main role in stimulating NK cells. Overall, the present study suggests the potential application of polysaccharides from P. sibiricum in functional foods and pharmacological industries.
- Subjects :
- Cell Survival
Cell
02 engineering and technology
Polysaccharide
Biochemistry
Mice
03 medical and health sciences
Sulfation
Immune system
Adjuvants, Immunologic
Polysaccharides
Structural Biology
medicine
Animals
Humans
RNA, Messenger
Molecular Biology
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
biology
Sulfates
Hydrolysis
Polygonatum
General Medicine
021001 nanoscience & nanotechnology
NKG2D
Killer Cells, Natural
RAW 264.7 Cells
medicine.anatomical_structure
Gene Expression Regulation
Perforin
chemistry
biology.protein
Cytokines
Signal transduction
0210 nano-technology
Cell activation
HT29 Cells
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 122
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....a327de2edba13d7b823fcecb67d9a3a9